CORE--VECTOR PRODUCTION

病媒核心的总体功能是向SCOR补助金的研究人员提供高质量的逆转录病毒载体，包括慢病毒载体。SCOR提案中的许多项目利用逆转录病毒载体作为实验工具，将特定基因导入造血细胞和其他培养细胞。载体核心将提供不同的载体载体和包装细胞系，以及与用于特定研究的最佳载体相关的不适当的技术支持。病媒核心将为项目6中的狒狒研究提供大量所需的病媒，并将在一个专门的设施中生产这些病媒，条件类似于生产人类使用的病媒。载体核心的设施可以被其他研究人员或载体核心工作人员使用，以产生适合人类使用的载体产生细胞。将解决生产具有复制能力的病毒的可能性的问题，并将为临床试验和实验室研究中使用的媒介进行此类病毒的分析。不能在核心进行的安全或毒性测试将根据需要承包给外部实验室。

The overall function of the Vector Core is to provide high quality retroviral vectors, including lentiviral vectors, to investigators of this SCOR grant. Many of the projects in this SCOR proposal utilize retroviral vectors as experimental tools to introduce specific genes into hematopoietic cells and other cultured cells. The Vector Core will provide different vector plasmids and packaging cell lines as well as inappropriate technical support relating to the best vectors to use for specific studies. The Vector Core will provide large quantities of vectors required for the baboon studies in Project 6, and will produce these vectors in a dedicated facility under conditions similar to those used for production of vectors for human use. The facilities of the Vector Core can be used by other investigators or Vector Core staff to generate vector-producing cells suitable for human use. Issues of the potential for production of replication-competent viruses will be addressed, and assays for such viruses will be performed for clinical trials and for vectors used in laboratory studies. Safety or toxicity assays that cannot be performed in the Core will be contracted to outside laboratories as needed.