Core--Pathology

病理学核心负责对注射泰勒病毒或用髓鞘抗原免疫以发展实验性自身免疫性脑脊髓炎的各种品系小鼠进行所有形态学评估。病理学核心位于罗德里格斯博士的研究实验室。病理学核心的主要重点是嵌入乙二醇甲基丙烯酸酯或Araldite中的塑料包埋切片。这些是评估脱髓鞘、髓鞘再生、脊髓萎缩和轴突缺失的标准测定。此外，将脑和脊髓切片包埋在石蜡中进行免疫细胞化学和原位杂交以检测病毒复制。冷冻切片用于神经丝蛋白的免疫荧光染色。T细胞和细胞因子表达和[3H]石房蛤毒素标记以评估轴突完整性。

The Pathology Core is responsible for all the morphological assessments of various strains of mice injected with Theiler's virus, or immunized with myelin antigens for development of experimental autoimmune encephalomyelitis. The Pathology Core is housed in Dr. Rodriguez's research laboratory. The major focus of the Pathology Core is primarily on plastic-embedded sections embedded in either glycol methacrylate or Araldite. These are the standard assays for assessment of demyelination, remyelination, spinal cord atrophy, and axonal loss. In addition, brain and spinal cord sections are embedded in paraffin for immunocytochemistry and in situ hybridization to detect virus replication. Frozen sections are used for immunofluorescence staining for neurofilament proteins. T cell and cytokine expression and [3H] saxitoxin-labeling to assess axonal integrity.