VARICELLA VIRUS LATENCY

猿猴的临床、病理、免疫学和病毒学特征 在灵长类动物中感染水痘病毒(SVV)实际上与 人类感染水痘带状疱疹病毒(VZV)。此外， SVV和VZV基因组在大小和结构上相似，表现出惊人的 它们的构型和DNA序列的同源性，并编码 抗原性相关的多肽。在潜伏期内，两种病毒都是 存在于多个神经节中，沿着它们各自的神经轴 宿主，至少有一个共同的基因被转录。最后，不同于任何 当前水痘潜伏期动物模型，VZV和SVV均重新激活 来自它们自然宿主的潜伏感染神经节。这些功能 为我们的假设提供了一个理论基础，即潜伏的物理状态 猴子的SVV与人类的VZV即使不完全相同，也不相似。人类 神经节在生命中是不能移除的，而猴神经节是潜伏的 感染了SVV的人很容易在生活中获得。因此，我们将使用 原位聚合酶链式反应和表达病毒的绿色荧光蛋白(GFP)鉴定 神经节细胞(S)，含有潜伏的SVV。另外，基于我们的 积累关于SVV全核苷酸序列的知识 基因组，我们将在一生中确定SVV的转录模式 潜伏感染的猴神经节中的基因。最后，我们将确定是否 SVV基因21(在潜伏期转录)的表达 猴神经节)是建立潜伏期所必需的。一个详细的 分析潜伏性猴水痘病毒的物理状态将导致 到旨在了解和预防人类水痘的实验 重新激活，是导致严重神经疾病的原因之一，特别是在 老年人口和免疫功能受损人口迅速增加。

Clinical, pathological, immunological and virological features of simian varicella virus (SVV) infection in primates are virtually identical to those of varicella zoster virus (VZV) infection in humans. Further, the SVV and VZV genomes are similar in size and structure, show striking homology in their configuration and DNA sequences, and encode antigenically related polypeptides. During latency, both viruses are present in multiple ganglia along the entire neuraxis of their respective hosts, and at least one common gene is transcribed. Finally, unlike any current animal model of varicella latency, both VZV and SVV reactivate from latently infected ganglia of their natural host. These features provide a rationale for our hypothesis that the physical state of latent SVV in monkeys is not similar, if not identical, to VZV in humans. Human ganglia cannot be removed during life, whereas monkey ganglia latently infected with SVV can be readily obtained during life. Thus, we will use in situ PCR and SVV-expressing green fluorescent protein (GFP) to identify the ganglionic cell(s) that harbors latent SVV. Also, based on our accumulating knowledge of the complete nucleotide sequences of the SVV genome, we will determine, during life, the transcriptional pattern of SVV genes in latently infected monkey ganglia. Finally, we will determine if the expression of SVV gene 21 (that is transcribed during latency in monkey ganglia) is required for establishment of latency. A detailed analysis of the physical state of latent simian varicella virus will lead to experiments designed to understand and prevent human varicella reactivation, a cause of serious neurologic disease, particularly in the rapidly increasing elderly and immunocompromised populations.