INTRODUCTORY PHASE

这项研究的目的是确定Zalk-8，一部小说 II型丝氨酸/苏氨酸激酶受体在颌面部生长和发育中的作用 发展。为了做到这一点，DNA构成了野生型 该受体的活性形式和截短形式连接到质粒中， 通过迷你预备队进行改造和隔离。1%琼脂糖凝胶 序列分析证实了这些扩增构建物的分离。 来自每种结构的信使核糖核酸正被用于显微注射研究 使用斑马鱼作为动物模型来帮助确定 这种新的受体正在开发中。潜在的配体相互作用可以 根据被视为注射的表型表达进行预测 鱼的发育，然而表征和隔离实际的 该受体的配体正在通过与 法国的调查人员。

The purpose of this study was to determine the role of zALK-8, a novel type II serine/threonine kinase receptor, in crainofacial growth and development. To do so, DNA constructs of wild type, constitutively active, and truncated forms of this receptor were ligated into plasmid, transformed, and isolated via minipreps. 1-% agarose gels followed by sequence analysis confirmed isolation of these amplified constructs. mRNA from each construct is being employed in microinjection studies using the zebrafish as an animal model to help determine the role of this novel receptor in development. Potential ligand interactions can be predicted based on the phenotypic expression seen as the injected fish develop, however characterization and isolation of the actual ligand for this receptor is being determined through collaboration with investigators in France.