WOUND HEALING IN THE CORNEAL STROMA

描述(申请人提供)：运动和形态变化 活着的小鼠角膜基质中的单个细胞将在 荧光显微镜。正常细胞和正常细胞的行为 邻里关系轻微，有界限，受伤也会随之而来。伤势将会 包括一种小的环状上皮清创或引入 在0.4毫米的圆周上通过离子导入产生氢离子，这两个圆周都是 可重现的。角质形成细胞和其他基质细胞之后将 利用转基因小鼠普遍表达荧光蛋白， 或用核或细胞质荧光染料染色。白细胞将会是 系统地用核染料标记。这些图像是由数字摄像机记录的 构建了用于分析的相机序列和时间推移序列。它的作用机制 轻微损伤后的细胞死亡、激活和再繁殖将是 进行了表征和分析。我们的目的是进一步研究将导致一个 阐明角膜的整个伤口愈合过程。它有一个 与外科伤口愈合和激光消融有直接关系 在屈光手术过程中造成的。因为细胞对细胞的反应 所有组织的损伤很可能是相似的，结果应该是广泛的 意义。

DESCRIPTION (provided by applicant): The movement and morphological changes of single cells in the corneal stroma of the living mouse will be observed under a fluorescence microscope. The behavior of normal cells as well as those in the neighborhood of a mild, circumscribed, injury will be followed. The injury will consist of a small circular epithelial debridement or the introduction of hydrogen ions by iontophoresis over a 0.4 mm circle, both of which are reproducible. The keratocytes and other stromal cells will be followed by the use of transgenic mice engineered to express fluorescent proteins ubiquitously, or by staining with nuclear or cytoplasmic fluorescent dyes. Leucocytes will be labeled with nuclear dye systemically. The images are recorded by a digital camera and time-lapse sequences are constructed for analysis. The mechanisms of cell death, activation and repopulation after a mild injury, will be characterized and analyzed. It is intended that further studies will lead to a clarification of the entire wound healing process in the cornea. It has an immediate relevance to the healing of surgical incisions and laser ablations made in the course of refractive surgery. Since the reactions of cells to injury in all tissues is likely to be similar, the results should have a wide significance.