CORE--IMMUNOLOGY AND MOLECULAR BIOLOGY

免疫学和分子生物学核心将提供免疫学和 病毒载量评估，以支持本计划的科学议程 项目对于生物行为干预研究，项目0002和 0004，CD 4 T细胞计数的病毒载量测量将在 基线和认知行为压力管理（CBSM）后， 如这些研究的实验设计中所定义的。项目0002 研究男同性恋和双性恋男性; 0004项目主要研究非洲人- 美国女人对于项目0005，从项目0002和 0004将在试验前和试验后进行实验室反应性研究， 干预以下免疫学试验将作为系列试验之一 在受试者身上采集的样本;淋巴细胞增殖试验（LPA） 对有丝分裂原植物血凝素的反应;爱泼斯坦抗体 巴尔病毒（EBV）、人疱疹病毒6型（HHV 6）和单纯疱疹 2型病毒（HSV 2）自然杀伤细胞（NKCC）对 肿瘤细胞靶，K562;淋巴细胞表型，使用4色血流 流式细胞术，测定CD 4，CD 8和亚群的数量和百分比 这些，使用对谱系，激活， 分化和粘附分子。在这些样品中，细胞溶解 使用基于抗穿孔素抗体的细胞内 将评估流式细胞术，并确定 通过荧光检测L-选择素（CD 62 L）、LFA-1（CD 11 a）和CD 56的表达 强度测量。项目0005还将包括体外 测定NK细胞的儿茶酚胺和细胞因子的作用的实验 这将由这个核心来完成。

The IMMUNOLOGY AND MOLECULAR BIOLOGY CORE will provide the immunology and viral load assessments in support of the scientific agenda of this Program Project. For the biobehavioral intervention studies, Projects 0002 and 0004, viral load measurements of CD4 T cell counts will be made at baseline and just after a cognitive behavioral stress management (CBSM), as defined in the experimental design for these studies. Project 0002 studies gay and bisexual men; Project 0004 studies predominantly African- American women. For Project 0005 selected subjects from Projects 0002 and 0004 will undergo a laboratory reactivity study both pre-and post- intervention. The following immunology assays will be one on the serial samples collected on subjects; lymphocyte proliferation assays (LPA) in response to the mitogen phytohemagglutinin (PHA); antibodies to Epstein Barr virus (EBV), human herpes virus type 6 (HHV6) and herpes simplex virus type 2 (HSV2) natural killer cell cytotoxicity (NKCC) against the tumor cell target, K562; lymphocyte phenotypes using 4-color flow cytometry, determining number and percent of CD4, CD8 and subsets of these, using monoclonal antibodies specific for lineage, activation, differentiation and adhesion molecules. On these samples cytolytic potential of NK cells using anti-perforin antibody-based intracellular flow cytometry will be assessed and determination made of degree of expression of L-selectin (CD62L), LFA-1 (CD11a) and CD56 by fluorescence intensity measurements. Project 0005 will also include in vitro experiments to measure the effects of catecholamines and cytokines of NK activity, that will be done by this core.