BIOTINYLATED ERYTHROCYTES IN PATIENTS WITH SICKLE CELL DISEASE

镰状红细胞受到许多重要的细胞变化和选择压力的影响。 在这项研究中，我们通过与标准51 CR标记进行比较来验证生物素RBC标记，并使用它来研究镰状细胞随着年龄的增长而发生的变化。 镰状红细胞的寿命比正常红细胞短得多，但两种标记对两种细胞类型的结果相同。 在回输后的最初几个小时内，不同数量的镰状红细胞（但不正常）在循环中消失。 生物素标记的镰状网织红细胞的数量在24小时后减少了50%，在48小时后减少了75%，每个细胞的网织红细胞数量逐渐减少。 标记的镰状细胞在回输后的前4至6天内表现出主要的密度增加，此后变化较小。 在最初几天后，一小群非常轻的标记镰状RBC的数量基本恒定。 在再输注后，在分离的生物素化镰状RBC中测定HbF含量，从而估计F细胞和非F细胞的寿命。 缺乏HbF的镰状B-RBC的寿命估计为约两周，而F细胞存活6至8周。

Sickle RBC are subject to a number of important cellular changes and selection pressures. In this study, we validated a biotin RBC label by comparison to the standard 51CR label, and used it to study changes that occur in sickle cells as they age. Sickle RBC had a much shorter lifespan than did normal RBC, but the two labels gave equivalent results for both cell types. A variable number of sickle, but not normal, RBC disappeared the circulation during the first few hours after reinfusion. The number of biotinylated sickle reticulocytes was decreased by fifty percent after 24 hours and 75% after 48 hours, with a gradual decrease in the amount of reticulum per cell. The labeled sickle cells exhibited major density increases during the first four to six days after reinfusion, with smaller changes thereafter. A small population of very light, labeled sickle RBC was essentially constant in number after the first few days. HbF content was determined in isolated biotinylated sickle RBC after reinfusion, allowing an estimate of lifespan for F cells and non-F cells. The lifespan of sickle B-RBC lacking HbF was estimated to be about two weeks, whereas F cells survived six to eight weeks.