ORAL MUCOSAL CELLS, CANDIDA AND CYTOKINE PRODUCTION

说明：本项目的具体目标包括：1）研究 原代口腔上皮细胞的促炎细胞因子分泌反应 培养到C.白色念珠菌攻击，包括IL-6和IL-8的产生， 确定与细胞因子应答相关的寄生虫因子; 2） 研究宿主细胞C.白色念珠菌的相互作用， 促炎细胞因子反应，包括直接接触的必要性， 甘露糖介导的信号传导在引发IL-6和IL-8应答中，以及 IL-6和IL-8的可能继发性干扰素产生信号的评估 合成;和3）确定是否可以通过 受C.白色念珠菌与口腔上皮细胞，包括 激活C。白念珠菌的吞噬作用和嗜中性粒细胞的增强作用 对上皮细胞分泌的促炎细胞因子的反应活性 用C.白色念珠菌

DESCRIPTION: The specific aims of this project include: 1) to study proinflammatory cytokine secretory responses of primary oral epithelial cell cultures to C. albicans challenge, including production of IL-6 and IL-8 and determination of parasite factors associated with cytokine response; 2) to study mechanisms of host cell-C. albicans interactions which lead to proinflammatory cytokine responses, including necessity for direct contact and mannose-mediated signaling in eliciting IL-6 and IL-8 responses, as well as assessment of possible secondary cytokine-generated signals for IL-6 and IL-8 synthesis; and 3) to determine whether neutrophil anti-fungal activity can be regulated by interaction of C. albicans with oral epithelial cells, including activation of C. albicans phagocytosis and enhancement of fungicidal neutrophil activity in response to the proinflammatory cytokines secreted by epithelial cells challenged with C. albicans.