REGULATION OF EXPRESSION OF BORRELIA BURGDORFERI BMPC

莱姆病是一种多系统的、由壁虱传播的慢性感染 伯氏疏螺旋体的持久性。勃氏假单胞菌 有能力在水库中的各种利基环境中繁殖 人类寄主以及肩部软体软体动物的媒介。初步 有证据表明，基因表达的差异是由 环境刺激在一种机制中允许细菌 快速适应感染期间发现的快速变化的环境和 病媒和宿主的疾病。我们和其他人描述了一个家庭 编码36.9-39.8 kDa同源基因的染色体基因(Bmpa-D) P39家族的脂蛋白。该基因的串联染色体定位 骨形态发生蛋白基因及其同源性和相互重叠的调控信号 与它们编码的蛋白质的潜在表面位置有关 除了它们明显受到环境刺激的上调外 这表明这些蛋白质可能对B。 伯格多费尔在不同环境中存活。在这个项目中，我们 建议研究环境刺激对bmpc，a的影响 我们首先描述的这个基因家族的成员，在体外和在 在小鼠和扁虱体内，以努力提高我们对 B.burgdorferi使用的机制使其能够持续和蓬勃发展 在载体和宿主中。我们努力的长期目标是改进 尽管缺乏对伯氏杆菌基因表达的理解 基因系统将促进这项工作。 本项目的具体目标是：1)确定抗BmpC单抗 与BmpD、BmpB和BmpB无交叉反应的多克隆抗体 并用它们来鉴定BmpC蛋白并确定其定位 和Burgdorferi 297中的脂肪作用；2)决定表达水平 以及不同体外和体外培养条件下bmpC的调控机制 活体条件；以及3)确定遗传组织和DNA B.burgdorferi、B.burgdorferi、B.burgdorferi中bmpC的结构和调控序列。 Afzelii和B.garinii基因种以及在B.burgdorferi 297中生长的 不同的体外和体内条件。后一项研究将 指示不同级别的转录是否次于 BmpC基因调控和结构DNA序列的变化。

Lyme disease is a multisystem, tick-borne chronic infection caused by the persistence of the spirochete Borrelia burgdorferi. B. burgdorferi has the ability to proliferate in a variety of niches in reservoir and human hosts as well as in the vector lxodes scapularis. Preliminary evidence indicates that differential gene expression resulting from environmental stimuli in one of the mechanisms that allows bacterial to adapt rapidly to fast changing environments found during infection and disease of vectors and hosts. We and others have described a family of chromosomal genes (bmpA-D) encoding homologous 36.9-39.8 kDa lipoproteins of the p39 family. The tandem chromosomal location of the bmp genes, their homology and overlapping regulatory signals together with the potential surface location of the proteins they encode in addition to their apparent up regulation by environmental stimuli suggests the hypothesis that these proteins may be important for B. burgdorferi survival in different environments. In this project, we propose to study the effects environmental stimuli have on bmpC, a member of this gene family first described by us, both in vitro and in vivo in mice and ticks, in an effort to improve our understanding of the mechanisms employed by B. burgdorferi to enable it to persist and thrive in vectors and hosts. The long-range goal of our efforts is to improve understanding of B. burgdorferi gene expression despite the lack of genetic systems that would facilitate this work. The specific aims of the current project are: 1) identify anti-BmpC Mab and polyclonal antibodies not cross-reactive with BmpD, BmpA and BmpB and use them to characterize BmpC protein and confirm its localization and lipidation in B. burgdorferi 297; 2) Determine levels of expression and mechanisms of regulation of bmpC under different in vitro and in vivo conditions; and 3) Determine genetic organization and DNA structural and regulatory sequences of bmpC in B. burgdorferi, B. afzelii and B. garinii genospecies and in B. burgdorferi 297 grown under different conditions in vitro and in vivo. The latter studies will indicate whether different levels of transcription are secondary to changes in the regulatory and structural DNA sequences of the bmpC gene.