WHOLE GENOME SEQUENCING OF PREVOTELLA INTERMEDIA

中间普雷沃氏菌也与I型糖尿病患者的牙周破坏和牙髓感染有关，包括根管感染、根尖周炎和根尖周病变。此外，这种细菌还与口腔外感染有关，包括儿童的急性扁桃体炎、细菌性气管炎和葡萄膜炎，最近还与NOMA（口腔炎）的发展有关，NOMA是一种破坏口面部和邻近结构的衰弱性传染病。许多中间青霉菌株具有血凝活性，有些菌株具有溶血活性。此外，增殖特征表明中间型巴斯德菌可能具有类似于葡萄球菌肠毒素A的“超抗原”活性。尽管P. intermedia与牙周病和其他疾病的关联及其诱导T细胞应答的能力，但在细胞和分子水平上对毒力特征的了解有限。有证据表明，中间变形杆菌是一种耐药性较高的牙周病原体，这在确定中间变形杆菌相关疾病的有效治疗方面提出了一个棘手的问题。因此，迫切需要开发遗传方法来增加我们对细胞/分子生物学和基因内容的理解，这将使研究人员能够开发新的治疗方法。确定完整的基因组序列是实现这些目标的最有效方法。我们建议对2.8 Mb进行测序。中间普雷沃氏菌临床分离株17，一种记录良好的强毒株。将使用TIGR目前使用的全基因组随机鸟枪法确定P. intennedia的完整基因组序列。

Prevotella intermedia has also been associated with periodontal breakdown in Type I diabetics and with endodontic infections including those of the root canal, apical periodotitis, and periapical pathosis. Additionally, this bacterium has been implicated in extra-oral infections that include acute tonsillitis, bacterial tracheitis, and uvuhtis in children and more recently in the development of NOMA (cancrun oris), a debilitating, infectious disease that destroys the oro-facial and neighboring structures. Many strains of P. intermedia posses hemagglutinating activity and some exhibit hemolytic activity. Furthermore, proliferation profiles suggest P. intermedia may possess "superantigenic" activity similar to staphylococcal enterotoxin A. Despite the association of P. intermedia with periodontal and other diseases and its ability to induce Tcell responses, only a limited knowledge of virulence features is available at the cellular and molecular levels. Evidence suggests that P. intennedia is one of the more drug resistant periodontal pathogens, which presents a formidable problem in determining effective treatments for the P. intermedia associated diseases. As a result, there is a critical need to develop genetic approaches to increase our understanding of the cellular/molecular biology and gene content, which will enable investigators to develop novel therapeutic approaches. A determination of the complete genome sequence represents the most effective approach towards realizing these goals. We propose to sequence the 2.8 Mb. Prevotella intermedia clinical isolate strain 17, a well-documented virulent strain. The complete genome sequence of P. intennedia will be determined using the whole genome random shotgun approach currently used at TIGR.