CORE--MORPHOLOGY

描述：（申请人描述）形态学核心的具体目的是为临床和实验GvHD影响的完整组织的结构检查提供技术援助、培训、专业知识和必要的服务和供应，并在目标组织水平上评估CD4肽类似物和相关工程蛋白的治疗干预效果。重点放在光学显微镜和专门的组织包埋和切片，以确保常规组织学，免疫组织化学和原位杂交的可用性。此外，将采用专门的超微结构和TUNEL染色来检测疾病发生和治疗反应的最早和最细微的变化。由于PI既是一名解剖病理学家，也是一名具有调查病理学背景的皮肤病理学家，因此在本核心中可以处理和评估皮肤和皮外目标组织。具体目标是：1。协助多肽结合的细胞和亚细胞定位（项目1）使用常规和专门的原位形态学方法评估所有小鼠组织的GvHD病理和肽反应（项目2）。提供形态学专业知识，以确认GvHD临床诊断，并在适当情况下提供临床活检标本的专业分析（项目3）。

DESCRIPTION: (APPLICANT'S DESCRIPTION) The specific aim of the Morphology Core is to provide technical assistance, training, expertise, and necessary service and supplies for structural examination of intact tissues affected by clinical and experimental GvHD, and to assess the efficacy of therapeutic intervention with the CD4 peptide analog and related engineered proteins at the level of target tissue. Emphasis is placed on light microscopy and specialized tissue embedding and sectioning necessary to insure the availability of conventional histology, immunohistochemistry, and in situ hybridization. In addition, specialized ultrastructure as well as TUNEL staining will be employed to enable detection of the earliest and most subtle changes of disease initiation and treatment response. Because the PI is both an anatomic pathologist as well as a dermatopathologist with a background in investigative pathology, both cutaneous and extracutaneous target tissues may be processed and evaluated in this Core. The specific aims are: 1. Assist in cellular and subcellular localization of peptide binding (Project 1) 2. Evaluate all murine tissues for GvHD pathology and peptide response using conventional and specialized in situ morphological approaches (Project 2). 3. Provide morphological expertise to confirm GvHD clinical diagnoses and to provide specialized analysis, where appropriate, of clinical biopsy specimens (Project 3).