PAPILLOMAVIRUS DNA REPLICATION--HOST CELL FACTORS

描述：乳头瘤病毒是人类重要的病原体，其生物学特性 仍然不能完全理解。这笔赠款的重点是解开 乳头瘤病毒DNA体外复制所需的蛋白质图谱。 在体外重组病毒DNA合成已经被用作 了解宿主复制机制的宝贵手段。是这样的 对SV40的实验提供了对基本DNA的巨大洞察力 哺乳动物细胞内的复制装置。纯净的曲目 在体外指导SV40 DNA合成的蛋白质是不够的 指导乳头瘤病毒DNA的体外合成。这一行动的主要目标是 格兰特的任务是分离和表征剩余的细胞活动 是复制乳头瘤病毒DNA所必需的。

DESCRIPTION: Papillomaviruses are important human pathogens whose biology is still incompletely understood. This grant is focused on unraveling the repertoire of proteins required for papillomavirus DNA replication in vitro. Reconstituting viral DNA synthesis in vitro has been used before as an invaluable means of understanding the host's replication machinery. Such experiments with SV40 have provided tremendous insight into the basic DNA replication apparatus within mammalian cells. The repertoire of purified proteins sufficient to direct SV40 DNA snthesis in vitro is not sufficient to direct papillomavirus DNA synthesis in vitro. The major goal of this grant is to isolate and characterize the remaining celllular activities required for papillomavirus DNA replication.