The interactome of the SMRT:GPS2:TBL1:HDAC3 complex

组蛋白脱乙酰酶(HDAC)是一种组蛋白修饰酶，在蛋白质折叠、细胞周期进程、细胞信号转导和新陈代谢等大量细胞过程中发挥作用。第一类HDACs：HDAC1、2和3均以多蛋白复合体形式存在于细胞核中，其活性和特异性由复合体的其他成分决定。II类HDAC包括HDAC4和5，它们不像I类HDAC那样具有高的固有催化活性，但通常与I类蛋白质以复合体的形式工作。该项目将涉及研究SMRT：GPS2：TBL1：HDAC3复合体的相互作用组，以使用BioID等技术识别关键的相互作用蛋白质，表征相互作用并解决使用核磁共振、结晶学或冷冻EM确定的任何相互作用的结构。

Histone deacetylases (HDACs) are histone modifying enzymes which play a role in a large number of cellular processes, such as protein folding, cell cycle progression, cell signalling and metabolism. The class I HDACs; HDAC1, 2 and 3 all exist in the nucleus as multi protein complexes in which the HDAC activity and specificity is determined by the other components of the complex. The class II HDACs include HDAC4 and 5, and these do not have high intrinsic catalytic activity like the class I HDACs but often operate in complexes together with the class I proteins. This project will involve looking at the interactome of SMRT:GPS2:TBL1:HDAC3 complex to identify key interacting proteins using techniques such as BioID, characterising the interactions and solving the structures of any interactions identified using NMR, crystallography or cryo-EM.