Suppression of wheat defences by Fusarium graminearum

将通过两个实验向学生介绍烟草和小麦的致病系统。实验1：将BSMV-V0 X载体与禾谷镰刀菌菌株、BSMV-V0 X载体进行比较，以及通过蛋白质印迹进行病毒检测和使用荧光定量MUG测定、组织平板接种和/或光学显微镜进行真菌检测。烟草叶片中Fg效应子功能的分析。使用完全随机化和重复的实验设计，将两种已经可用的BSMV：Fg效应子构建体与两种对照构建体（BSMV：MCS和BSMV：iLov）一起沿着接种到烟草植物上，以探索Fg效应子的过表达如何影响该非宿主物种的镰刀菌感染。实验2. Fg GUS和Fg tri 5突变体对小麦穗感染的分析。采用重复成对试验设计，小麦品种。Bobwhite将用对照BSMV：iLov构建体接种或模拟接种，然后7天后，整个小麦穗将用Fg GUS菌株或Fg tri 5突变体接种。Fg症状发展将每天目测评估，并且在第7天将解剖小麦小穗以探索和相互比较具有和不具有病毒的每种Fg菌株的定殖程度。

Two experiments will be done to introduce the student to the tobacco and wheat pathosystems, the two F. graminearum strains, the BSMV-VOX vector as well as virus detection by Western blotting and fungus detection using a fluorescent quantitative MUG assay, tissue plating and/or light microscopy.Experiment 1. Analysis of Fg effector function in tobacco leaves. Using a fully randomised and replicated experimental design two already available BSMV:Fg effector constructs will be inoculated onto tobacco plants along with two control constructs (BSMV:MCS and BSMV:iLov) to explore how the over-expression of Fg effectors affects Fusarium infection of this non-host species. Experiment 2. Analysis of wheat spike infection by the Fg GUS and Fg tri5 mutant. Using a replicated pair wise experimental design, the wheat cv. Bobwhite will either be inoculated with the control BSMV:iLov construct or mock inoculated and then 7 days later the entire wheat spike will be inoculated with either the Fg GUS strain or the Fg tri5 mutant. Fg symptom development will be visually assessed on a daily basis and the at day 7 the wheat spikelets will be dissected to explore and inter-compare the extent of colonisation of each Fg strain with and without the virus.