POTASSIUM CHANNEL ACTIVATION BY VOLATILE ANESTHETICS

描述：（改编自申请人的摘要）这第一个奖项 建议通过以下方式检查基线K+渗漏通道的激活情况 挥发性麻醉剂具体目标是：（1）确定 通道激活通过检查挥发性麻醉剂在一系列 浓度改变通道特性，除了研究基本的 该通道类型的生理学和药理学;（2），识别和 表征挥发性麻醉剂作用的结合位点， 检查它们阻断内源性信使的能力， 和卤代非麻醉剂的作用能力 对通道和抑制异氟烷的作用，和（3）， 确定这些通道在调节神经元兴奋性中的作用 和突触传递。研究将在小脑进行 使用全细胞破裂贴片和切除贴片的颗粒神经元 技术在培养的神经元和薄片上。

DESCRIPTION: (Adapted from the applicant's abstract) This FIRST award proposal is to examine the activation of baseline K+ leak channels by volatile anesthetics. Specific aims are (1) to determine the mechanism of channel activation by examining how volatile anesthetics in a range of concentrations alter channel properties, in addition to study the basic physiology and pharmacology of this channel type; (2), to identify and characterize the binding sites at which volatile anesthetics act by examining their ability to occlude actions of endogenous messengers such as arachidonic acid and the ability of halogenated non-anesthetics to act on the channels and to inhibit the actions of isoflurane, and (3) to determine the role of these channels in modulating neuronal excitability and synaptic transmission. Studies will be carried out in cerebellar granule neurons using whole cell ruptured patch and excised patch techniques on neurons in culture and in thin slices.