VAGAL-MEDIATED CYTOKINES IN SLEEP CIRCUITRY

描述（改编自申请人摘要）：食物摄入过量， 外周感染性攻击与非快速眼的增加有关 运动睡眠（NREMS）迷走神经及其诱导的细胞因子上调 似乎在某种程度上调节了这些睡眠反应。然而，无论行动 迷走神经中的电位诱导脑中的细胞因子上调， 中枢回路将迷走神经传入与睡眠和细胞因子增加联系起来 生产仍然未知。迷走神经介导的脑内细胞因子增加 在脑干和下丘脑这两个区域观察到mRNA水平 参与调节NREMS。细胞类型的神经解剖定位 参与这些增加的细胞因子mRNA水平的评估，在一系列的 实验测试对以下假设进行了评价：（1）行动 迷走神经传入的电位是负责的，（2）来自迷走神经的神经回路 迷走神经传入投射到孤束核，通过 臂旁外侧核至下丘脑前部和视前内侧 区负责。最后，假设这个回路中的神经元 正在以迷走神经介导的方式上调其细胞因子mRNA水平， 通过逆向标记特定的神经投射进行测试。映射 脑干和下丘脑内迷走神经介导的脑内细胞因子 将为功能性神经解剖学提供新的见解， NREMS规范。

DESCRIPTION (adapted from applicant's abstract): Excessive food intake and peripheral infectious challenge are associated with increases in non-rapid eye movement sleep (NREMS). The vagus and vagal-induced upregulation of cytokines seems to mediate these sleep responses, in part. However, whether action potentials in the vagus induce cytokine upregulation in the brain and the central circuit tying vagal afferents to increased sleep and cytokine production remains unknown. Vagal-mediated intracerebral increases in cytokine mRNA levels have been observed in the brainstem and hypothalamus, two areas implicated in regulating NREMS. Neuroanatomical localization of the cell types involved in these increased cytokine mRNA levels are evaluated in a series of experimental tests. The following hypotheses are evaluated: (1) action potentials in vagal afferents are responsible and (2) the neural circuitry from the vagal afferent projection to the nucleus of the solitary tract through the lateral parabrachial nucleus to the anterior hypothalamus and medial preoptic area is responsible. Finally, the hypotheses that neurons within this circuitry are upregulating their cytokine mRNA levels in a vagal-mediated fashion is tested by retrogradely labeling specific neural projections. Mapping the vagal-mediated intracerebral cytokines within the brainstem and hypothalamus will provide new insights into the functional neuroanatomy that underlies the regulation of NREMS.