CROSSTALK OF AT1A RECEPTOR SIGNALING

AT 1受体对血压很重要。 为了理解血管紧张素11与受体结合的总体效应，受体的信号传导机制特别令人感兴趣。 本提案涉及：具体目标1：研究位于AT受体羧基尾部的5个酪氨酸对G蛋白偶联和/或激活的重要性。假设-AT 1受体羧基尾部的酪氨酸是蛋白偶联和/或激活所必需的。具体目的2：研究位于AT 1受体羧基尾的5个酪氨酸对酪氨酸激酶活性的意义。 假设-AT受体羧基尾部的5个酪氨酸突变不会破坏受体与细胞内酪氨酸激酶蛋白的偶联。

The AT1 receptor is important for blood pressure. The signaling mechanisms of the receptor is of particular interest in order to understand the overall effect of angiotensin 11 binding to the receptor. The proposal herein addresses: Specific Aim 1: To investigate the importance of the five tyrosines located on the carboxyl tail of the AT receptor for G-protein coupling and/or activation. Hypothesis - The tyrosines on the carboxyl tail of the AT1 receptor are necessary for -protein coupling and/or activation. Specific Aim 2: To investigate the significance of the five tyrosines located on the carboxyl tail of the AT1 receptor for tyrosine kinase activity. Hypothesis - The mutation of the five tyrosines on the carboxyl tail of the AT receptor does not disrupt the coupling of the receptor to intracellular tyrosine kinase proteins.