The roles of a poorly characterized protease in rescuing the genome from protein linked DNA breaks

蛋白质连接的DNA断裂（PDB）在基因组代谢中产生了重大挑战，当它们不被修复时，基因组变得不稳定并可能导致细胞死亡。操纵该途径以抑制PDB的修复已广泛用于癌症治疗。在正常代谢中共价结合DNA的蛋白质在某些情况下可以依赖于专门的酶来去除它们。该项目将研究一种特性不佳的酵母酶在DNA修复过程中的作用，并鉴定可能成为抗癌药物有用靶点的人类直系同源物。成功的候选人将接受先进的分子生物学技术，酵母和人类遗传学，哺乳动物细胞培养和DNA修复分析的培训。

Protein linked DNA breaks (PDBs) create major challenges in genome metabolism, when they are not repaired genomes become unstable and can cause cell death. Manipulation of this pathway to inhibit repair of PDBs has been used widely in cancer treatment. Proteins that covalently bind DNA in normal metabolism can, in certain circumstances, rely on specialised enzymes for their removal. The project will characterise the roles of a poorly characterised yeast enzyme during DNA repair and identify human orthologues that may become a useful target for anti-cancer drugs. The successful candidate will receive training in advanced molecular biology techniques, yeast and human genetics, mammalian cell culture, and DNA repair assays.