Development of optical projection tomography for biomedical imaging

这个博士项目关注的是在亚毫米到厘米范围内对“介观”生物样品进行三维光学成像的新技术的开发和应用。这样的技术可以允许生物过程在活的完整生物体（例如果蝇、线虫和斑马鱼）中原位成像，并且可以促进对于机械切片来说太精细或太精细的完整组织标本的高分辨率3-D组织病理学。该项目将特别利用光学投影断层扫描（OPT），这是一种介观成像技术，特别适合于较大的样品，并具有广泛部署相对低成本的设备用于现实世界应用的巨大潜力。博士研究将在光子集团实验室内进行，并与生命科学系和医学院的同事合作。3D成像的新方法将应用于生物样品，包括活斑马鱼（胚胎-成体），用于疾病过程的临床前成像，例如与癌症，炎症和细菌感染相关的疾病过程，以及大块完整组织样品的3D组织病理学，包括小鼠胰腺和脑组织的清除样品。

This PhD project concerns the development and application of novel techniques for 3-D optical imaging of "mesoscopic" biological samples in the sub-mm to cm range. Such techniques can permit biological processes to be imaging in situ in live intact organisms, such as drosophila, nematodes and zebrafish, and can facilitate high resolution 3-D histopathology of intact tissue specimens that are too fine or too delicate for mechanical sectioning. The project will particularly exploit optical projection tomography (OPT), which is a mesoscopic imaging technique that is particularly suited for larger samples and has great potential for wide deployment of relatively low cost devices for real-world applications. The PhD research would be undertaken within the Photonics Group laboratories and also in collaboration with colleagues from the Department of Life Sciences and the Faculty of Medicine. Novel approaches for 3-D imaging will be applied to biological samples, including live zebrafish (embryo-adult) for preclinical imaging of disease processes, e.g. associated with cancer, inflammation and bacterial infection, and 3-D histopathology of bulk intact tissue samples, including cleared samples of mouse pancreas and brain tissue.