Changes in gene expression and alternative polyadenylation in response to Hypoxia

本项目将研究选择性多聚腺苷化（APA）在细胞应激转录组反应中的作用。APA是在转录过程中使用替代的聚腺苷化位点来获得具有不同3' utr的mRNA转录物。这些3' utr通常包含影响转录本定位、功能和稳定性的信号序列。因此，APA可以极大地影响转录组对特定细胞状态的响应。HEK293T细胞将受到一系列环境损伤，如活性氧和紫外线辐射。细胞核和细胞质mRNA将从细胞中分离出来，并使用RNA-Seq测序。生物形态分析将评估APA谱的任何变化，并确定转录组的关键变化，然后进行进一步的研究。BBSRC优先领域：生物科学的系统方法数据驱动生物学[b]， ENWW

This project will investigate the role of alternative polyadenylation (APA) in the transcriptomic response to cellular stress. APA is the use of alternative polyadenylation sites during transcription to give mRNA transcripts with varying 3'UTRs. These 3'UTRs often contain signal sequences which influence the transcripts localisation, function and stability. Therefore, APA can greatly affect the transcriptome in response to a specific cellular state. HEK293T cells will be subjected to a range of environmental insults such as reactive oxygen species and Ultraviolet radiation. Nuclear and cytoplasmic mRNA will be isolated from the cells and sequenced using RNA-Seq. Bioformatic analysis will evaluate any changes in the APA profile and identify key alterations to the transcriptome which will then undergo further investigation.BBSRC Priority Areas:Systems approaches to the biosciencesData driven biologyWUB, ENWW