EXCITATORY AMINO ACIDS AND RECEPTORS IN SOMESTHESIS

拟议的研究计划植根于谷氨酸是 a/由外周神经元建立的第一突触的主要神经递质 中枢神经系统中的神经纤维 由于周围神经 具有不同功能特性的纤维释放相同的 可能涉及突触后机制。 这些可以包括 不同类型的传入纤维优先终止于 不同类型的谷氨酸受体。 第一部分建议 研究将测试这种可能性。 周围神经损伤可导致味觉亢进，即反应增强 “疼痛”刺激，这可能是由谷氨酸拮抗剂阻断。 的 可能是痛觉过敏的基础的神经元过度兴奋可能是 这可以用谷氨酸盐的密度或分布的变化来解释 背角神经元中的受体。 这是第二部分的内容。 提出研究计划，以确定。 这里计划的研究没有 到目前为止，由于缺乏足够的 方法. 电镜免疫金的改进 使现在有可能比较准确定性和定量 损伤和对照脊髓中谷氨酸受体的研究进展 材料

The proposed research plan is rooted in the notion that glutamate is a/the major neurotransmitter t the firs synapse established by peripheral nerve fibers in the central nervous system. Since peripheral nerve fibers with different functional characteristics release the same transmitter, postsynaptic mechanisms may be involved. These may include preferential termination of different types of afferent fibers onto different types of glutamate receptors. The first part of the proposed research will test this possibility. Peripheral nerve injury may result in hypergeusia, i.e. enhanced response to "painful" stimuli, that may be blocked by glutamate antagonists. The neuronal hyperexcitability that may underlie hyperalgesia may be explained by changes in the density or distribution of glutamate receptors in dorsal horn neurons. This is what the second part of the proposed research plans to determine. The research planned here has not been possible until now at the microscopic level for lack of adequate methods. A modification of electron microscopic postembedding immunogold makes now possible to compare with accuracy qualtitive and quantitive aspects of glutamate receptors in injured and control spinal cord material.