OCULAR CONTROL OF MELATONIN REGULATION--ACTION SPECTRUM

光感受器系统将光刺激转换为神经元信号， 内分泌、昼夜节律和光的治疗效果是未知的。 的 这项研究的总体目标是确定受体机制， 介导光的这些生物学和治疗效果。 实现 这一目标，一个行动谱（不同的相对有效性， 用于引发生物反应的波长）， 帮助识别光调节的感光系统 人体内的褪黑激素 然后，该作用谱可以用作 研究作用光谱和相关感光细胞的工具 参与昼夜节律调节和光疗法的系统。 拟议研究的具体目的是确定一项行动 光谱用于光诱导的血浆褪黑激素抑制使用8 单色波长（10 mm半峰带宽）在400到760之间 NM. 这些波长对急性抑制 将在正常志愿者中研究褪黑激素。 注量-响应曲线 覆盖从10到10的5对数单位光子密度范围<14><19> 将<2>为每个角膜水平建立光子/cm 波长 将通过绘制 半饱和刺激的倒数（sigma<-1>） 测试了8个波长。 该作用谱将阐明范围和 将光刺激传递到 人体神经内分泌和昼夜节律系统。

The photoreceptor system that transduces light stimuli for neuro- endocrine, circadian, and therapeutic effects of light is unknown. The overall aim of this research is to determine the receptor mechanism that mediates these biological and therapeutic effects of light. To achieve this aim, an action spectrum (the relative effectiveness of different wave lengths for eliciting a biological response) will be established to help identify the photoreceptor system for light regulation of the hormone melatonin in humans. This action spectrum may then be used as a tool for investigating the action spectrum and related photoreceptor system involved in circadian regulation and light therapy. The specific aim of the proposed research is to determine an action spectrum for light-induced plasma melatonin suppression using 8 monochromatic wavelengths (10mm half-peak bandwidths) between 400 to 760 NM. The effects of these wavelengths on the acute suppression of melatonin will be studied in normal volunteers. fluence-response curves covering a 5 log unit photon density range from 10<14> to 10<19> photons/cm<2> at the level of the corneal will be established for each wavelength. an action spectrum will be constructed by plotting the reciprocal of the half-saturation stimulus (sigma<-1>) from each of the 8 wavelengths tested. This action spectrum will clarify the range and peak sensitivity of the receptor system that transmits photic stimuli to the human neuroendocrine and circadian system.