MOTOR CORTEX AND THALAMOCORTICAL NETWORK IN LOCOMOTION

描述(改编自申请人的摘要) 这项建议旨在1)了解已确定的 运动皮质网络中的元素和运动行为，以及2) 了解脑内丘脑-皮质关系的调控机制 运动系统完好无损。所有数据将从训练有素的猫那里获得 在平坦的地面上以不同的速度走进实验室， 上坡和下坡，用重物固定在前肢上，克服障碍 以及走在水平梯子的扁平横梁上。两国之间的关系 已鉴定的皮质神经元、皮质分离传出神经元和推测 中间神经元会在不同的运动行为中被记录下来。在……里面 此外，这些已识别的神经元的活动将被记录下来 与丘脑皮质投射神经元的活动同步，以及 它们之间的相互作用将使用互相关法进行分析，网址为“ 休息“和运动时。将特别强调阐明 控制功能连接的强度和专一性的规则 正在研究的丘脑皮质和皮质神经元的数量。两大 问题将被解决：1)不同的活动在多大程度上 已识别的运动皮质传出神经元的类别(例如，S1，SII， 皮质丘脑、皮质脊髓等)和假定的抑制性中间神经元 在不同的条件下，与运动有关的不同？是属性 更好地与皮质层、形态细胞类型或 轴突的去向？2)VL的活动模式是什么 不同条件下丘脑皮质投射神经元的运动 条件以及这些模式与大脑皮层神经元的模式相比如何？ VL投射之间的突触联系有什么特点 神经元和皮质受体神经元以及这些联系是如何在 不同的运动条件？

DESCRIPTION (adapted from applicant's abstract) This proposal is aimed at 1) understanding the relationship between identified elements within the motor cortical network and locomotion behavior, and 2) understanding the mechanisms governing thalamo-cortical relations in the intact, functioning motor system. All data will be obtained from cats trained to walk inside an experimental chamber on a flat surface at different speeds, uphill and downhill, with weights attached to forelimbs, overcoming obstacles and walking on flat cross-pieces of a horizontal ladder. The relationship of identified cortico-cortical neurons, corticofugal efferent neurons and putative interneurons will be recorded during different locomotion behaviors. In addition, the activity of these identified neurons will be recorded simultaneously with the activity of thalamo-cortical projection neurons, and interactions among them will be analyzed using cross correlation methods at " rest" and during locomotion. Special emphasis will be placed on elucidating rules governing the strength and specificity of functional connections among the population of thalamo-cortical and cortical neurons under study. Two major issues will be addressed: 1) To what extent does the activity of different classes of identified motor cortex efferent neurons (e.g., S1, SII, collosal, corticothalamic, corticospinal, etc) and putative inhibitory interneurons differ in relation to locomotion under different conditions? Are properties better associated with cortical layer, morphological cell type or the destination of the axon? 2) What are the activity patterns of VL thalamo-cortical projection neurons during locomotion under different conditions and how do these patterns compare with those of cortical neurons? What are the characteristics of the synaptic linkages between VL projection neurons and cortical recipient neurons and how do these linkages change under different conditions of locomotion?