SEQUENCING THE MOUSE GENOME

怀特黑德/麻省理工学院基因组研究中心迫切希望为到2003年完成小鼠基因组草稿序列和到2005年完成完成基因组序列的目标做出贡献。在为期三年的项目中，我们计划生产大约25%的小鼠基因组的序列覆盖率。具体地说，我们将对总计750Mb的BAC克隆进行测序--包括630Mb的草稿序列和120Mb的成品序列，每个碱基大约5美分，每个碱基大约12美分。将根据对小鼠群落的生物学兴趣和对小鼠-人类的比较研究选择区域进行测序。在项目过程中，我们还建议(I)测试涉及BAC克隆池的基因组测序策略，以及(Ii)与新的试点中心开展技术转移活动。

The Whitehead/MIT Center for Genome Research is eager to contribute to the goals of completing the sequence of the mouse genome in draft form by 2003 and in finished form by 2005. During the three-year project, we propose to produce sequence coverage of approximately 25 percent of the mouse genome. Specifically, we will sequence BAC clones totaling 750 Mb-consisting of 630 Mb of draft sequence at approximately 5 cent per base and 120 Mb of finished sequence at approximately 12 cent per base. Regions will be selected for sequencing on the bases of biological interest for the mouse community and for comparative mouse-human studies. In the course of the project, we also propose to (i) test strategies for genomic sequencing involving pooling of BAC clones and (ii) engage in technology transfer activities with new pilot centers.