600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER

需要一个高场核磁共振光谱仪来支持圣安东尼奥德克萨斯大学健康科学中心正在进行的生物分子结构研究。如果获得资助，提议的光谱仪将致力于生物导向的研究，这需要最先进的高场核磁共振仪器和相对较长的连续仪器时间。拟议的工具将大大加强对七个主要用户的研究。这一多样化用户群体的共同特征包括：1)现场高场生物分子核磁共振仪器的迫切需求；2)致力于解决当代生物化学基本问题的研究问题；3)NIH或NSF支持的研究项目，具有重要的核磁共振成分。拟议的文书将大大促进下列不同领域的研究：(1)细胞因子-细胞因子研究和蛋白- RNA相互作用（Hinck），(2)氨基酸取代对细胞色素c的结构、稳定性和折叠途径的影响（Nall）， (3) PDZ结构域在介导蛋白质定位中的作用（Masters），(4)伴侣蛋白GroEL的顶端结构域的灵活性和功能（Horowitz），(5)磷酸水解酶mutT家族的底物特异性和机制（Barnes），(6)通过H-D功能标记检测细胞色素氧化酶-细胞色素c相互作用（Robinson）；(7)核心组蛋白尾部结构域相互作用（Hansen）。该请求是合理的，因为圣安东尼奥迫切需要高场核磁共振仪器，能够执行三重共振和脉冲场梯度实验，这对我们用户群的数据收集需求至关重要。拟议的文书得到大学的大力支持。所要求的40万美元资金将与圣安东尼奥德克萨斯大学健康科学中心的45.4万美元资金相结合。大学还将提供资源来支付仪器安装、维护和核磁共振设施管理人员的工资。该仪器将成为生物分子结构分析机构中心的核心组成部分，这是一个大分子结构测定的综合中心，占地超过11,000平方英尺的新建成的研究空间，位于德克萨斯大学圣安东尼奥校区健康科学中心的联合健康科学大楼。该仪器将作为共享资源运行，为位于圣安东尼奥的德克萨斯大学健康科学中心和南德克萨斯研究界服务。

A high field NMR spectrometer is needed to support ongoing studies of biomolecular structure at the University of Texas Health Science Center at San Antonio. If funded, the proposed spectrometer will be dedicated to biological-oriented research that requires state-of-the-art high-field NMR instrumentation and relatively long, continuous blocks of instrument time. The proposed instrument will greatly enhance the research of seven major users. Common features of this diverse group of users include: 1) An immediate need for on site high-field biomolecular NMR instrumentation, 2) Research problems dedicated towards solving fundamental problems in contemporary biochemistry, and 3) NIH or NSF supported research programs, having a significant NMR component. Research in the following diverse areas will be significantly advanced by the proposed instrument: (1) Cytokine-cytokine research and Protein- RNA interactions (Hinck), (2) The effects of amino acid substitutions on the structure, stability, and folding pathways of cytochrome c (Nall), (3) The role of PDZ domains in mediating protein localization (Masters), (4) Flexibility and function in the apical domain of the chaperonin GroEL (Horowitz), (5) Substrate specificities and mechanism of the mutT family of phosphohydrolases (Barnes), (6) Cytochrome exidase-cytochrome c interactions as probed by H-D functional labeling (Robinson), and (7) Core histone tail domain interactions (Hansen). The request is justified by the urgent need for high field NMR instrumentation in San Antonio with the ability to perform triple resonance and pulsed field gradient experiments that are essential to the data collection needs of our user base. The proposed instrument has the strong endorsement of the University. The requested funds of $400,000 will be combined with $454,000 from sources within the University of Texas Health Sciences Center at San Antonio. The University will also provide the resources to cover instrument installation, maintenance, and the salary for the NMR facility manager. The instrument will become a core component of the Institutions Center for Biomolecular Structure Analysis, a comprehensive center for macromolecular structure determination, which occupies over 11,000 ft2 of newly completed research space in the Allied Health Sciences Building on the University of Texas Health Science Center at San Antonio campus. The instrument will operate as a shared resource serving the University of Texas Health Science Center at San Antonio and the South Texas research community.