MACROMOLECULAR STRUCTURE

大分子结构共享资源(MSSR)由X射线结晶学(X-ray)组成 和核磁共振(核磁共振)实验室。X射线结晶学和核磁共振是非常重要的 阐明三维结构和研究大分子的互补方法 互动。他们共同为癌症中心成员提供了全面的方法来 了解与癌症相关的生物大分子在正常和患病状态下的功能 分子水平。癌症中心的调查人员将得到建议和协助，利用这些先进的 确定癌症相关生物大分子的结构并研究其结构的技术 X射线导向器与其他大分子和潜在治疗剂的相互作用 和核磁共振实验室，分别为P.John Hart博士和Andrew P.Hinck博士。它的X射线成分 MSSR包括一个Art Robbins结晶机器人和两个最先进的Rigaku-MSSR X射线数据 收集系统。该仪器提供了进行所有现代X射线衍射的全部能力 适用于获得高质量的蛋白质、核酸的三维结构 以及他们的情结。MSSR的核磁共振组件包括最先进的布鲁克光谱仪 配备了工作在500、600和700兆赫的高灵敏度低温探头。这台仪器 提供使用N、C和H标记的现代核磁共振实验的全部功能 适用于三维溶液结构的获取和研究 与其他大分子和潜在治疗剂的相互作用。MSSR可供访问 更广泛的癌症中心成员由受过博士培训的技术经理亚历克斯·泰勒、博士和乌达亚组成 分别为X射线实验室和核磁共振实验室提供指导 从样品准备到解释和展示结果的整个过程。MSSR提供了一个 一系列全面的方法，用于可视化和功能表征与癌症相关的疾病 生物大分子。

The Macromolecular Structure Shared Resource (MSSR) is comprised of the X-ray crystallography (X-ray) and the Nuclear Magnetic Resonance (NMR) laboratories. X-ray crystallography and NMR are highly complementary methods for elucidating three-dimensional structures and for studying macromolecular interactions. Together, they provide Cancer Center members with comprehensive methodologies to understand how cancer-related biological macromolecules function in normal and diseased states at the molecular level. Cancer Center investigators will be advised and assisted in utilizing these sophisticated technologies to determine structures of cancer-related biological macromolecules and to investigate their interacfions with other macromolecules and with potential therapeutic agents by the Directors of the X-ray and NMR laboratories, P. John Hart, PhD and Andrew P. Hinck, PhD, respectively. The X-ray component of the MSSR includes an Art Robbins crystallization robot and two state-of-the-art Rigaku-MSSR X-ray data collecfion systems. The instrumentation provides full capabilities for conducting all modern X-ray diffraction experiments and is suitable for obtaining high quality three-dimensional structures of proteins, nucleic acids and their complexes. The NMR component of the MSSR includes state-of-the-art Bruker spectrometers equipped with high sensitivity cryoprobes operating at 500, 600, and 700 MHz. The instrumentafion provides full capabilities for conducting modern NMR experiments with N, C, and H labeled macromolecules and is suitable for obtaining three-dimensional solution structures and investigating interactions with other macromolecules and potential therapeutic agents. The MSSR is made accessible to the broader Cancer Center Membership by PhD-trained technical managers, Alex Taylor, PhD, and Udayar llangovan, PhD, for the X-ray and NMR laboratories, respectively, who provide guidance at each step in the process, from sample preparation to interpretation and presentation of results. The MSSR provides a comprehensive array of methodologies with which to visualize and functionally characterize cancer-related biological macromolecules.