MACROMOLECULAR STRUCTURE

大分子结构共享资源（MSSR）由X射线晶体学（X-ray） 以及核磁共振实验室。X射线晶体学和核磁共振是高度 阐明三维结构和研究大分子的补充方法 交互.他们共同为癌症中心成员提供全面的方法， 了解癌症相关的生物大分子如何在正常和疾病状态下发挥作用， 分子水平。癌症中心的研究人员将得到建议和协助， 确定癌症相关生物大分子结构并研究其 与其他大分子和潜在的治疗药物的相互作用，由X射线的导演 和NMR实验室，P. John哈特，博士和Andrew P. Hinck，博士。的X射线成分 MSSR包括一个阿特罗宾斯结晶机器人和两个最先进的Rigaku-MSSR X射线数据 收集系统。该仪器提供了进行所有现代X射线衍射的全部能力 实验，适用于获得高质量的蛋白质，核酸， 及其复合物。MSSR的NMR组件包括最先进的布鲁克光谱仪 配备有在500、600和700 MHz下操作的高灵敏度冷冻探针。仪器 提供进行现代NMR实验的全部功能，其中N、C和H标记 适用于获得三维溶液结构和研究 与其他大分子和潜在治疗剂的相互作用。MSSR可供以下人员使用： 更广泛的癌症中心成员由博士培训的技术经理，亚历克斯泰勒，博士，和Udayar llangovan博士分别为X射线和NMR实验室提供指导， 从样品制备到结果解释和呈现的整个过程。MSSR提供了 一系列全面的方法，用于可视化和功能性表征癌症相关的 生物大分子

The Macromolecular Structure Shared Resource (MSSR) is comprised of the X-ray crystallography (X-ray) and the Nuclear Magnetic Resonance (NMR) laboratories. X-ray crystallography and NMR are highly complementary methods for elucidating three-dimensional structures and for studying macromolecular interactions. Together, they provide Cancer Center members with comprehensive methodologies to understand how cancer-related biological macromolecules function in normal and diseased states at the molecular level. Cancer Center investigators will be advised and assisted in utilizing these sophisticated technologies to determine structures of cancer-related biological macromolecules and to investigate their interacfions with other macromolecules and with potential therapeutic agents by the Directors of the X-ray and NMR laboratories, P. John Hart, PhD and Andrew P. Hinck, PhD, respectively. The X-ray component of the MSSR includes an Art Robbins crystallization robot and two state-of-the-art Rigaku-MSSR X-ray data collecfion systems. The instrumentation provides full capabilities for conducting all modern X-ray diffraction experiments and is suitable for obtaining high quality three-dimensional structures of proteins, nucleic acids and their complexes. The NMR component of the MSSR includes state-of-the-art Bruker spectrometers equipped with high sensitivity cryoprobes operating at 500, 600, and 700 MHz. The instrumentafion provides full capabilities for conducting modern NMR experiments with N, C, and H labeled macromolecules and is suitable for obtaining three-dimensional solution structures and investigating interactions with other macromolecules and potential therapeutic agents. The MSSR is made accessible to the broader Cancer Center Membership by PhD-trained technical managers, Alex Taylor, PhD, and Udayar llangovan, PhD, for the X-ray and NMR laboratories, respectively, who provide guidance at each step in the process, from sample preparation to interpretation and presentation of results. The MSSR provides a comprehensive array of methodologies with which to visualize and functionally characterize cancer-related biological macromolecules.