AMIDE LINKED DNA--HYBRIDZATION BEHAVIOR AND TEMPLATE DIRECTED SYNTHESIS

该项目研究了DNA主链修饰在双链稳定性中的作用，以及全主链修饰的低聚物作为生物信息传递模型系统的效用。合成了一系列中性骨架DNA类似物，并对其杂交行为进行了系统的研究。这些修饰的低聚物将进一步用作指导互补底物组装的模板。竞争实验将揭示相对于天然DNA，修饰的低聚物作为模板和底物的相对效率。这项研究将导致1)扩展PI开发的方法，通过固相合成技术组装酰胺链DNA类似物。2)更深入地了解对复制、转录和翻译至关重要的分子识别事件的影响因素；3)在简单的体外模板定向复制系统的发展方面取得进展；4)为芝加哥州立大学的教师和学生在最先进的实验室中创造生物医学相关的研究机会。

This project investigates the role of DNA backbone modifications in duplex stability and the utility of fully backbone-modified oligomers as model systems for biological information transfer. A series of neutral- backbone DNA analogues are being synthesized and their hybridization behavior systematically studied. These modified oligomers will be further utilized as templates for directing the assembly of complementary substrates. Competition experiments will reveal the relative efficiency of the modified oligomers as templates and substrates relative to native DNA. This research will result in 1) the extension of methodology developed by the PI for assembly of amide-linked DNA analogues by solid-phase synthetic techniques. 2) greater understanding of the factors contributing to the molecular recognition events critical to replication, transcription, and translation, 3) progress in the development of simple in vitro template-directed replicating systems, and 4) the creation of biomedical-related research opportunities for Chicago State University faculty and students in a state of the art laboratory.