Isolation and characterisation of bacteriophages against avian Escherichia coli carrying multi-drug resistant (MDR) plasmids

一般背景：携带MDR质粒的大肠杆菌等细菌病原体的抗生素耐药性被认为是对全球健康的最大威胁之一，估计每年造成70万人死亡。不幸的是，控制这些病原体的策略受到抗生素耐药性增加的阻碍，因此需要研究替代治疗方法。噬菌体是一种只感染和杀死细菌的病毒，自20世纪80年代以来，在西方被认为是抗生素化疗的替代方案。项目背景：噬菌体除了通过直接感染杀死病原体外，还可以作为一种工具，通过噬菌体附着在AMR质粒的接合皮利上来减少MDR质粒的携带。我们有DTP轮换的一部分的初步数据（Maluping，Atterbury，巴罗，Jones 2017）。鉴定出4株特异性靶向大肠杆菌的噬菌体。大肠杆菌含有来自15个噬菌体分离物的初始库的抗性质粒pFlacTn3。（Maluping，R.，旋转数据（巴罗等人，2017）。这些噬菌体可用于特异性靶向携带AMR细菌的质粒并减少AMR以支持AMR细菌感染的治疗。作为这些细菌的一部分的初始EM表征表明，这些噬菌体都是加尾的，并且最有可能被分类为肌尾病毒科的成员（Maluping，R.，旋转数据（巴罗等人，2017）。

General background: Antibiotic resistance in bacterial pathogens such as Escherichia coli carrying MDR plasmids is considered to be one of the greatest threats to global health, and responsible for an estimated 700,000 deaths per year. Unfortunately, strategies for the control of such pathogens have been impeded by increasing antibiotic resistance, so there is a need to investigate alternative treatments. Bacteriophage (phage) are viruses that infect and kill only bacteria, and have been mooted as an alternative to antibiotic chemotherapy in the West since the 1980s.Project background: In addition to killing pathogens by direct infection, phage can also be used as a tool to reduce the carriage of MDR plasmids using phage attach to conjugative pili of the AMR plasmids. We have preliminary data a part of the DTP rotation (Maluping , Atterbury, Barrow, Jones 2017). Four phage were identified phage which specifically targeted E. coli contain the resistance plasmid pFlacTn3 from an initial pool of 15 phage isolates. (Maluping, R., rotation data Barrow et al. 2017). These phage could be used to specifically target the plasmid bearing AMR bacteria and reduce AMR to support treatment of AMR bacterial infections. Initial EM characterization as part of these bacteria indicated that these phage were all tailed and most likely classified as members of the Myoviridae (Maluping, R., rotation data Barrow et al. 2017).