Engineering molecular membrane pores

固态核磁共振小型项目：其目的是在一个简单的生物研究从蛋白质表达和纯化，样品制备通过生物物理分析和表征的连续性的味道。第1-2周：重组蛋白将被过表达、纯化并在有和没有NMR标记的情况下表征;第3-4周：将制备具有重构蛋白的模型膜并将其转化为定向双层片段，即双胞。将通过固态NMR研究脂质膜，以获得蛋白质的15 N光谱。第5-6周：将进行光谱模拟以模拟双胞中的蛋白质取向，并将这些光谱与实验光谱进行比较以评估蛋白质取向。

Solid state NMR mini-project: The objective is to give in a nutshell a flavour of the continuity in biological research from protein expression and purification, sample preparation through biophysical analysis and characterisation. Week 1-2: Recombinant protein will be overexpressed, purified and characterised with and without NMR labels; Week 3-4: Model membranes with reconstituted protein will be prepared and converted into oriented bilayer fragments, bicelles. The lipid membranes will be studied by solid state NMR to obtain a 15N spectrum form the protein. Week 5-6: Spectral simulations will be carried out to simulate the protein orientation in bicelles and these will be compared to experimental spectra to assess protein orientation.