MUSCLE PROTEIN BIOPHYSICS VIA ATOMIC FORCE MICROSCOPY

肌肉生理学和肌肉病理学是NIAMS和NHLBI等ICS使命下的重要研究课题。在与NIAMS研究人员的持续合作中，原子力显微镜(AFM)和相关的生物物理方法正被应用于研究肌肉蛋白质，如肌蛋白(一种由3-4丙二醛组成的巨大肽链)和星云蛋白(600-900 kDa)。这项工作包括对应用于Titin研究的AFM和激光光学陷阱(LOT)纳米技术的全面综述，以及由此产生的对Titin生物物理特性的理解以及对肌肉生理学和病理学的影响。被称为PEVK区域的Titin基序的分子弹性的AFM测量和解释也在继续。原子力显微镜对星云结构和模块折叠能量的研究以及数据解释和原子力显微镜仪器的进步是其他活动领域和 成就。

Muscle physiology and muscle pathologies are important research subjects under the missions of ICs such as NIAMS and NHLBI. In a continuing collaboration with NIAMS researchers, atomic force microscopy (AFM) and related biophysical methods are being applied to investigate muscle proteins such as titin (a massive peptide chain of 3-4 MDa) and nebulin (600-900kDa). The work includes coauthoring a comprehensive review of AFM and laser optical trap (LOT) nanotechnologies as applied to titin research and the resulting understandings on titin biophysical properties and implications for muscle physiology and pathology. AFM measurements and interpretations of the molecular elasticity of a titin motif, termed PEVK region, is also continuing. AFM investigations of nebulin structure and modular folding energetics and data interpretations and AFM instrument advancement are other areas of activity and achievements.