To develop a new core facility in Proteomics

描述(由申请者提供)：作为全校努力的一部分 发展基因组学/蛋白质组学，建议添加最先进的 蛋白质阵列1系统，与基于质量的多肽分析/测序相结合以 我们的兽医DNA核心设施。该仪器将被安置在 最近翻修了兽医学院的核心设施，以及 由一个主要用户委员会监督。主要用户组代表5个 主要学术/研究中心和7个不同的学院系 兽医、医学院和牙医学院 位于校园一到两个街区的区域内。该设施将用于 大部分(90%)由拥有50多项NIH拨款和16项其他拨款的用户组提供 联邦/非联邦拨款。主要用户群体的研究兴趣为 侧重于细胞和分子生物学的基础和临床方面， 包括转基因，生殖细胞生物学，组蛋白的发育方面， 血红蛋白和免疫球蛋白基因表达，精子细胞中的基因表达 成熟，胚胎发育。肌肉发生，线粒体应激信号，性 肝脑药物代谢、致畸、细胞转化的二倍体 神经肌肉和造血疾病，癌症遗传学，遗传性疾病 遗传性代谢性疾病与动物模型基因治疗的发展。 所有列出的用户都有对高敏感性蛋白质的需求 测序。大约有8个组有高通量分析的要求 系统，其中约9个有分析齐聚物的要求 含有已知和未知蛋白质的混合物，约有12种蛋白质具有 用于鉴定和定位磷酸化、糖基化位点 以及N端子和内部处理。该设施将是 由经验丰富的生物化学家/分子生物学家主持 在蛋白质化学方面，他目前经营着一个质谱仪实验室。

DESCRIPTION (provided by applicant): As part of school-wide efforts in developing genomics/proteomics, it is proposed to add a state-of-the-art protein array1 system, coupled to a mass-based peptide analysis/sequencing to our Vet DNA Core Facility. The instrument will be housed and operated in the recently renovated core facility at the School of Veterinary Medicine, and supervised by a Committee of major users. The major user group represents 5 major academic/research centers and 7 different departments of School of Veterinary Medicine, School of Medicine, and School of Dental Medicine, all located within a one to two block area of the campus. The facility will be used mostly (90 percent) by the user group with more than 50 NIH grants and 16 other federal/non-federal grants. The research interests of the major user group are focused on the basic as well as clinical aspects of cell and molecular biology, including transgenesis, germ cell biology, developmental aspects of histone, hemoglobin and immunoglobin gene expression, gene expression during sperm cell maturation, embryogenesis. myogenesis, mitochondrial stress signaling, sexual dimorpism in hepatic brain drug metabolism, teratogenesis, cell transformation, neuromuscular and hematopoeitic diseases, cancer genetics, genetic disorders of inherited metabolic diseases and development of gene therapy in animal models. All of the listed users have requirement for high sensitivity protein sequencing. About 8 groups have requirements for the high throughput analysis system, about 9 of them have requirements for analyzing oligomeric complexes containing a mixture of known and unknown proteins, about 12 have requirements for the identification and localization of phosphorylation, glycosylation sites as well as both N-terminal as well as internal processing. The facility will be run by an experienced biochemist/molecular biologist with extensive experience in protein chemistry, who is currently running a mass spectrometry laboratory.