Purchase of Technai 12 TEM/STEM Electron Microscope

描述（由申请人提供）：此申请是一个资金请求 帮助购买一台飞利浦TECHANI 12 TEM/Stem电子显微镜。的 仪器将取代Philips EM 400仪器，该仪器已使用20多年 并且其使用寿命即将结束。它也缺乏最先进的技术 新仪器的特点，这是非常需要的快速收集 高分辨率的数据。Technai 12 TEM/杆将进入井中 连续22年。核心设施拥有所有必要的咨询 集团，收费制度和人员的日常运作所需的共享 仪器 该设施在杰克格里菲斯博士的指导下， 研究人员都在亚特兰大和美国各地的需要可视化的 它们的核酸和核酸-蛋白质复合物。该设施及其 操作是非常独特的：在过去的10年里，60篇涉及EM的论文 在我们提供EM的高知名度期刊上发表 其他国家卫生研究院资助的私家侦探今年（2000-2001年）， 与美国33个NIH资助的实验室合作。Technai 12 TEM/股骨柄 更高的分辨率，新的成像模式，捕捉图像的能力， 直接传输到计算机，并且这种仪器能够被我们的 通过高速互联网连接， 都是至关重要的，以继续这一重要的地方和国家 操作

DESCRIPTION (provided by applicant): This application is a request for funding to help purchase a Philips Techani 12 TEM/Stem electron microscope. The instrument will replace a Philips EM400 instrument, which is over 20 years old and is reaching the end of its useful life. It also lacks state of the art features of the new instruments, which are greatly needed for rapid collection of high-resolution data. The Technai 12 TEM/Stem will go into a well continuously for 22 years. The core facility has all of the required advisory groups, charge systems, and personnel for daily operation required for a shared instrument. This facility under the direction of Dr. Jack Griffith serves NIH funded investigators both at UNC and across the US in need of the visualization of their nucleic acid and nucleic acid-protein complexes. This facility and its operation is highly unique: in the past 10 years, 60 papers involving EM have been published in high profile journals resulting from our providing EM expertise to other NIH funded P.I.?s and this year (2000-2001) we are engaged in work with 33 NIH funded laboratories across the US. The Technai 12 TEM/Stem with grater resolution, new imaging modes, the ability to capture images directly to a computer, and the ability for this instrument to be used by our collaborators at their home institutions via a high speed internet connection are all essential to the continuation of this important local and national operation.