2- AND 3-D IMAGING OF METABOLIC ACTIVITION OF BETA CELLS

在可行性研究中，快速冷冻胰腺的高分辨率(50微米)二维和三维成像显示，在葡萄糖挑战下，来自NADH(P)H和黄蛋白(FP)还原以及血红蛋白浓度增加的独特定位信号。这一结果鼓励比较与葡萄糖和亮氨酸滴定的响应图像，以区分线粒体和胞浆NADH(P)H的响应。氧化还原变化的时间分辨成像提供了进一步的区别；预计胞液响应将先于线粒体(FP)响应，这些时间分离将通过葡萄糖/亮氨酸的比较得到证实。β细胞反应的鉴定将通过组织切片的组织病理学来确认，组织切片经过处理并从最后一次光学扫描的组织块中切下。预计这些Snap冷冻成像研究将为未来基于方法2(飞行荧光)和方法3(近红外经腹成像)的体内β细胞功能的微创光学评估提供理论基础。

High resolution (50 mu)2- and 3-dimensional imaging of snap frozen pancreas shows in feasibility studies distinctive localized signals from NADH(P)H and flavoprotein (Fp) reduction and hemoglobin concentration increases in response to glucose challenges. This result encourages comparison of images in response to titrations with glucose and leucine in order to differentiate the response of mitochondrial and cytosolic NADH(P)H. A further distinction is afforded by time-resolved imaging of redox changes; it is expected that cytosolic responses will precede mitochondrial (Fp) responses and these time separations will be confirmed by glucose/leucine comparisons. Identification of beta cell responses will be confirmed by histopathology of tissue sections processed and microtomed from the tissue block which the last optical scan is made. It is expected that these snap frozen imaging studies will afford a rationale for future minimally invasive optical evaluation of beta cell function in vivo base upon Method 2, Flying Fluorescence and Methods 3, NIR Transabdominal Imaging.