DIFFERENTIALLY EXPRESSED GENES IN OLFACTORY NEUROGENESIS

描述（改编自申请人摘要）：本研究的目标是 描述在早期和中期阶段发生的分子变化， 成年小鼠嗅感受器神经元的神经发生。 嗅 受体神经元是为数不多的神经元群体， 在成年脊椎动物中持续替换。 虽然有几个基因的例子 已知嗅觉受体神经元谱系中的表达变化， 仅代表必须发生的变化的一小部分。 进展 重组DNA技术现在可以同时鉴定大的 没有先验序列信息的差异表达基因的数量。 因此，研究者的目的是利用这项技术来鉴定mRNA， 在大量的同步神经发生过程中， 嗅觉受体神经元 这将包括识别mRNA， 不同的丰富之间的早期和中期阶段的过程中， 神经发生 这些实验有可能：1）确定一个 参与嗅觉受体神经发生的基因的显著部分 神经元; 2）识别调节神经发生的信号通路; 3）扩展 嗅觉受体神经元谱系中细胞类型的标志物的数量; 和4）鉴定谱系中先前未知的细胞类型。 在嗅觉中， 这些实验对嗅觉器官的胚胎发育有一定的启示 上皮，用于嗅癌，用于再生，以及 嗅上皮 这些实验也暗示了 一般成年哺乳动物的神经发生，包括利用这一知识， 开发神经退行性疾病和创伤性损伤的干预措施， 神经系统

DESCRIPTION (adapted from applicant's abstract): The goal of this research is to describe molecular changes that occur during early and intermediate steps in the neurogenesis of olfactory receptor neurons of adult mice. The olfactory receptor neurons are one of the few populations of neurons that undergo continuous replacement in adult vertebrates. While a few examples of gene expression changes in the olfactory receptor neuron lineage are known, they represent only a minute fraction of the changes that must occur. Advances in recombinant DNA technology now make it possible to simultaneously identify large numbers of differentially expressed genes without a priori sequence information. Therefore, the investigator's aim is to use this technology to identify mRNAs that increase in olfactory epithelia during massive, synchronous neurogenesis of olfactory receptor neurons. This will include identifying mRNAs that are differentially abundant between early and intermediate stages in the process of neurogenesis. These experiments have the potential to: 1) identify a significant fraction of the genes involved in neurogenesis of olfactory receptor neurons; 2) identify signaling pathways that regulate neurogenesis; 3) expand the number of markers of cell types in the olfactory receptor neuron lineage; and 4) identify previously unknown cell types in the lineage. Within olfaction, the experiments have implications for embryonic development of the olfactory epithelium, for olfactory carcinomas, for regeneration, and aging processes in the olfactory epithelium. The experiments also have implications for neurogenesis in adult mammals in general, including using this knowledge to develop interventions in neurodegenerative disorders and traumatic injury to the nervous system.