CORE--TRANSGENIC MOUSE

转基因小鼠核心的目的是提供两个密钥 为计划项目成员提供科学支持服务。其核心是 将与一名首席研究员密切合作 具体的PO1项目，提供专业技术和设备来生产 转基因小鼠。对于一些项目，核心将使用特殊的基因 进行转基因的背景。其次，核心将提供 再次与密切合作，提供产生零突变小鼠的服务 项目调查员。与其他常见的核心支持不同 服务，我们决定以“现收现付”的方式组织这项服务 基础，即要求的资金仅用于维护核心模型 单个项目建议的将是 主要由特定人员产生并将由该特定人员支付 项目。在一些不同的方面提供指导和建议 生成鼠标模型所需的技术将由 核心人员。此外，还介绍了Res.Spec.II由核心支付费用 将执行这些程序中的关键步骤(例如，发展ES 细胞，显微注射)。该组织的主要考虑因素 结构是一方面，实质性的“动手”时间 单个项目需要参与，例如长达1.5年的 一种零突变的小鼠，通常比平时需要更多的时间和精力 正常的工作日。另一方面，很有可能 关键步骤只有完全由某人有效地执行 精通这类技术的致力于这种技术并对其有经验的鼠标模型将会有 将在项目1(米努博士)、项目2(沃伯顿博士)和 项目4(格罗芬博士)。基于我们最近的几个项目 调查会议，很可能项目3(德林奇博士)将 有必要建立小鼠模型(S)(零突变和转基因)。这个 核心领导人(Heisterkamp博士)，他在 这两种类型的老鼠模型的生成，都将批判性地评估 每个建筑的设计，并将参与到教学中 人事部。

The purpose of the Transgenic Mouse Core is to provide two key scientific support services to members of the Program Project. The core will, in close collaboration with the principal investigator of a specific PO1 project, provide the expertise and equipment to produce transgenic mice. For some projects, the Core will use a special genetic background to make transgenics. Secondly, the core will provide services to generate null-mutant mice, again in close collaboration with the project investigator. Unlike other commonly available core support services, we have decided to organize this service on a "pay-as-you-go" basis i.e. funds are requested only to maintain the coreouse models which the individual Project Project projects propose to make will be largely generated by personnel of and will be paid by that specific project. Instruction in and advice on some of the different technologies necessary to generate mouse models will be provided for by core personnel. In addition, the Res. Spec. II paid for by the Core will perform the critical steps in these procedures (e.g., growing ES cells, microinjection). A prime consideration for this organizational structure is on the one hand, the substantial "hands-on" time involvement required for an individual project, e.g. up to 1.5 years for a null-mutant mouse, often reuiring more time and effort than usual for a normal working day. On the other hand, it is very likely that critical steps will only be performed efficiently by someone fully dedicated to and experienced in such techniques. Mouse models will have to be generated inproject 1 (Dr. Minoo), project 2 (Dr. Warburton) and project 4 (Dr. Groffen). Based onseveral of our more recent project investigatory meetings, it is likely that project 3 (Dr. DeRynch) will have a need to develop mouse model(s) (null-mutant and transgenic). The Core leader (Dr. Heisterkamp) who has many years of experience in the generation of both types of mouse models, will critically evaluate the design of each construct and will be involved in the teaching of personnel.