Light sheet fluorescence microscopy for high speed 3-D imaging: applications to the study of heart disease and cancer cell invasion

光片荧光显微镜使活细胞具有低光漂白和光毒性的高速3D荧光成像。该项目将开发新型光片显微镜技术，以高速3D方式研究活细胞，并在多孔板中进行细胞的3D筛选。该项目的第一部分将开发一种新型的倒置光片荧光显微镜系统，该系统使用自适应光学元件，用于高速3D成像的快速重新聚焦，以及用于低速但高空间分辨率成像的第二种操作模式。这将使心肌细胞的结构能够在高分辨率下进行研究，随后在高速3D中对两个数据集进行精确的空间配准。现有的斜平面显微镜装置也将在项目中用于研究癌细胞在3D培养物中的侵袭，项目的一个重要部分将是开发和加速图像采集，预处理和分析管道。

Light sheet fluorescence microscopy enables high speed 3D fluorescence imaging of live cells with low photobleaching and phototoxicity. This project will develop novel light sheet microscopy technology to study live cells in high speed in 3D and to perform 3D screening of cells in multiwell plates. The first part of the project will develop a novel inverted light sheet fluorescence microscope system using adaptive optic elements for rapid refocusing for high speed 3D imaging as well as a second mode of operation for lower speed but high spatial resolution imaging. This will enable the structure of cardiomyocytes to be studied at high resolution and subsequently in 3D at high speed with accurate spatial registration of the two datasets. The existing oblique plane microscope setup will also be used during the project to study the invasion of cancer cells in 3D cultures and a significant part of the project will be to develop and accelerate the image acquisition, preprocessing and analysis pipeline.