Human Natural Killer Cells

描述(申请人提供)：这项研究的具体目的是研究人类天然受体、配体和功能的某些方面 杀伤(NK)细胞。我们尤其希望： 1.研究抑制性和溶血性NK免疫的形成 Synapse。 2.确定靶细胞上激活多重裂解的配体 人NK细胞上的受体。 3.检测人蜕膜淋巴细胞对NK细胞和自然杀伤细胞的影响 杀伤T(NKT)细胞，包括它们的频率、基因和功能 (特别是细胞因子的产生及其在植入和 怀孕)，并研究杀伤细胞与 免疫球蛋白样受体(KIR)单倍型变异与再妊娠 损失。 4.检查关于a)KIR3D的标识的KIR 区别识别人类白细胞抗原-B2705和人类白细胞抗原-B2702和b)产生 识别异常KIR2DL4和KIR2DL5的单抗 用于研究它们的多态、配体和功能。

DESCRIPTION(provided by applicant): The specific aims of this research are to examine some aspects of the receptor, ligands, and functions of human Natural Killer (NK) cells. In particular, we wish: 1. To study the formation of both the inhibitory and the lytic NK immunologic synapse. 2. To identify the ligands on target cells for the multiple lysis activating receptors on human NK cells. 3. To examine human decidual lymphocytes with respect to NK cells and Natural Killer T (NKT) cells, including their frequency, genotype, and functions (especially production of cytokines and their possible role in implantation and pregnancy) and to examine the possible relationship between Killer cell Immunoglobulin-like Receptor (KIR) haplotype variations and recurrent pregnancy loss. 4. To examine KIR with respect to a) identification of the KIR3D that differentially recognizes HLA-B2705 and HLA-B2702 and b) generation of monoclonal antibodies recognizing the unusual KIR2DL4 and KIR2DL5 that could be used in studies of their polymorphism, ligands, and functions.