CORE--IMMUNOLOGY FACILITY

本提案描述了NYU CFAR免疫学核心将提供的设施和服务。本核心的主要目的是为艾滋病研究界的研究人员提供HIV中和活性的细胞免疫功能测定、HIV结构蛋白特异性人单克隆抗体（mab）的供应、啮齿动物单克隆抗体的生产以及检测性能和人单克隆试剂生产方面的培训。该核心的服务可供纽约大学艾滋病研究社区、纽约大学其他领域的研究人员（如纽约大学癌症中心的成员）、纽约大学以外的nih支持的研究人员以及世界各地需要单克隆抗体进行有价值的艾滋病毒相关项目的科学家使用。在Core进行的检测包括（但不限于）几种抗原或有丝分裂原之一的淋巴细胞增殖，外周血单个核细胞（PBMCs）的体外抗体产生，CD8细胞对病毒生长的抑制以及细胞毒性T淋巴细胞检测。其他服务包括测量血清中抗体介导的HIV中和活性和使用几种测定方法制备单克隆抗体。此外，我们还培训了许多来自纽约大学和其他美国和国际研究机构和大学的人员进行这些不同的检测。Core还为世界各地的研究人员提供了70多种抗hiv的人类单克隆抗体。单克隆抗体不收费（因为在大学和国外收费的复杂性），但已经建立了免疫和中和试验的收费系统。未来的建议是基于过去10年CFAR资助发展的专业知识和不断变化的需求，以及当地、国家和国际研究人员的需求。

This proposal describes the facilities and services to be provided by the Immunology Core of the NYU CFAR. The primary purpose of this Core is to provide investigators within the AIDS research community access to assays of cellular immune function measurements of HIV neutralizing activity, supplies of human monoclonal antibodies (mAbs) specific for structural proteins of HIV, production of rodent mAbs, and training on the performance of assays and production of human monoclonal reagents. The services of this core are available to the NYU AIDS research community, to NYU researchers in other fields (such as members of the NYU Cancer Center), to NIH-supported researchers outside of NYU, and to those scientists around the world who need mAbs for meritorious HIV- related projects. The assays performed in the Core include (but are not limited to) lymphoproliferation to one of several antigens or mitogens, in vitro antibody production by peripheral blood mononuclear cells (PBMCs), inhibition of virus growth by CD8 cells, and cytotoxic T lymphocyte assays. Additional services include measurement of Ab- mediated HIV neutralizing activity in sera and preparations of mAbs using several assays. In addition, we have trained many individuals, from NYU and other American and international research institutes and universities in the conduct of these various assays. The Core also provides more than 70 anti-HIV human mAbs to researchers around the world. No charge is made for the mAbs (because of the complexity of billing outside of the university and outside of the country), but a charge-back system has been established for immunologic and neutralization assays. The proposal for the future is based on the expertise developed and the evolving needs over the past 10 years of CFAR funding and upon the needs of local, national and international investigators.