CORE--MORPHOLOGY

Morphology Core的主要目标是通过促进皮肤样本显微分析技术的使用，提高新的P&F研究人员和已建立的皮肤研究人员的多功能性和生产力。这将通过维护经验证的试剂（抗血清）库、执行免疫染色方案、筛选新的抗血清、指导/优化原位杂交方案筛选转基因小鼠的组织学皮肤异常以及提供皮肤病理学协助来实现。该核心将提供一个开拓/培育环境，在经验丰富的团队的指导下，最初尝试并实现尖端的显微技术。该核心将实施具有更高灵敏度的新技术的引入，并为更大的SDRC用户组产生途径特异性技术（整合素组、凋亡标记物、信号转导探针和抗血清）的集群，并加速从基于同位素的方案向非同位素方法的转换。核心将提供最先进的包埋，切片，染色，定量形态测定，摄影布局和打印的显微标本在及时和低成本的基础上。Morphology Core的另一个主要功能是获取具有准确人口统计学和诊断数据的临床来源的人体样本。对于进行皮肤相关项目的研究人员来说，皮肤病理学和伤口愈合样本的大型集中库的持续可用性是一个关键组成部分和宝贵的资源。主任和所有研究助理将为P&F研究人员、受资助的SDRC研究人员及其博士后研究员、研究生和实验室人员提供用于皮肤分析的显微镜技术方面的教学协助和建议。分子遗传学和表型核心之间的协同作用将每天发生，将速冻组织递送至分子遗传学核心或从表型核心生成动物样本和/或培养细胞。

The primary objective of the Morphology Core is to enhance the versatility and productivity of both new P&F investigators and established skin investigators by facilitating access to techniques directed at the microscopic analysis of skin samples. This will be accomplished by maintaining banks of proven reagents (antisera), executing immunostaining protocols, screening new antisera, instructing/optimizing in situ hybridization protocols screening transgenic mice for histologic skin abnormalities, and providing dermatopathologic assistance. This core will provide a pioneering/nurturing environment where cutting edge microscopic techniques are initially tried and achieved under the guidance of an experienced team. The core will implement the introduction of new techniques with greater sensitivities and generate clusters of pathway- specific techniques (integrin panels, apoptosis markers, signals transduction probes and antisera) to the greater SDRC user group, and hasten the conversion from isotopic based protocols to non-isotopic methods. The Core will render state-of-the-art embedding, sectioning, staining, quantitative morphometry, photographic layout and printing for microscopic specimens on a timely and low-cost basis. Another prime- function of the Morphology Core will be to procure clinical-derived human samples with accurate demographic and diagnostic data. The continue availability of large, centralized bank of dermatopathologic and wound healing samples is a critical component and invaluable resource for investigators who conduct skin-related projects. The Director and all research assistants will supply teaching assistance and advice in microscopic techniques useful for skin analysis for P&F investigators, Funded SDRC investigators and their post-doctoral fellows, graduate students, and laboratory personnel. Cooperative interactions among the Molecular Genetics and Phenotype Cores will occur on a daily basis snap frozen tissues are delivered to the Molecular Genetics Core or animal samples and/or cultured cells are generated from the Phenotype Core.