FROM GENOMIC SEQUENCES TO PROTEIN STRUCTURE/FUNCTION

此计划项目应用程序的目标是分配结构和 对基因组开放阅读框的蛋白质产物起作用， 没有具有已知三维结构的同系物， 功能与现行做法不同，申请人及其 项目领导人建议开展全面、协调一致的努力， 使用高通量晶体学方法， 原子级结构的功能。选择的互补DNA 流感嗜血杆菌基因和所指出的其他微生物将 克隆用于在大肠杆菌中表达和纯化 为了获得足够量的蛋白质， 结晶和X射线晶体学和NMR 3D结构 决心。

The goal of this Program Project application is to assign structure and function to the protein products of genomic open reading frames for which there are no homologues with known three dimensional structure and function. In a departure from current practice, the applicant and his project leaders are proposing to mount a comprehensive, concerted effort, using high throughput crystallographic approaches, to determine protein function from atomic level structure. Complementary DNA from selected Hemophilus influenzae genes, and other microorganisms as indicated, will be cloned for expression in Escherichia coli and purification of the protein products in order to obtain sufficient quantities of protein for crystallization and X-ray crystallographic and NMR 3D structure determinations.