DNA determinants for chromatin modifiers in yeast

描述(由申请人提供) 需要理解的一个重要的生物学问题是如何 依赖于ATP的染色质重塑复合体针对特定的 推动者。因此，这项研究提案的一个具体目的是澄清 复杂招募的分子机制(S)。它正在迅速地成为 最重要的是理解这一目标过程，因为有一个 与染色质重塑相关的科学发现激增 对癌症起作用。为了促进对复杂招聘的理解， 萌芽酵母酿酒酵母将被用作模式生物。 鉴于其遗传上的易操纵性，酵母是一种吸引人的有机体，可以用它来 研究染色质结构修饰剂的潜在分子机制。 此外，酿酒酵母的完整基因组已经测序，这 允许使用全基因组DNA微阵列技术。这个 通过这项技术获得的初步数据将允许全球 鉴定与特定染色质修饰剂相关的启动子 将导致以下问题：哪些启动子序列是必需的 招聘指定的修改活动？这是最基本但最关键的 机械性问题可以通过启动子缺失分析来回答， 异源启动子的构建及启动子的详细分析 染色质结构。一旦这些络合物的分子靶向被牢牢地 了解到，未来可以提出实验，以确定是否参与 依赖三磷酸腺苷的染色质重塑复合体与癌症。

DESCRIPTION (provided by applicant) A significant biological question that needs to be understood is how ATP-dependent chromatin remodeling complexes are targeted to specific promoters. Therefore, a specific aim of this research proposal is to elucidate the molecular mechanism(s) of complex recruitment. It is rapidly becoming paramount to understand this targeting process since there has been an explosion of scientific discoveries linking altered chromatin-remodeling function with cancer. To facilitate the understanding of complex recruitment, the budding yeast, Saccharomyces cerevisiae, will be used as a model organism. Given its genetic tractability, yeast is an appealing organism with which to study the underlying molecular mechanisms of modifiers of chromatin structure. Moreover, the complete genome of S. cerevisiae has been sequenced and this allows for the ability to employ genome-wide DNA microarray technology. The preliminary data obtained by this technique will allow for the global identification of promoters associated with specific chromatin modifiers which will lead to the following question: what promoter sequences are required for recruitment of a given modifying activity? This fundamental but critical mechanistic question can be answered by promoter deletion analysis, construction of heterologous promoters, and detailed analysis of promoter chromatin structure. Once the molecular targeting of these complexes is firmly understood, future experiments can be proposed to determine the involvement of ATP-dependent chromatin remodeling complexes with cancer.