DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAIN

描述：(改编自《调查者摘要》)：实验模型 将导致精心控制的大脑缺氧水平，由直接 测量组织微循环中的氧气水平，然后 复氧期。这些模型将包括由 动脉低氧(FiO2减少；高流量低氧)和出血性 低血压伴双侧颈动脉闭塞(低流量低氧)。这个 细胞功能障碍和/或损伤的机制(S)将在两个 细胞和分子水平。细胞外谷氨酸和谷氨酸水平的变化 多巴胺将通过体内微透析进行持续监测。大脑 将对组织进行筛选，以寻找可能在表达上发生变化的多个基因 作为缺氧/缺血发作和复氧的结果。这 将使用放大的反义RNA(Arna)技术进行，以及基因 选择进行分析将对应于被认为是关键的蛋白质 多巴胺-谷氨酸相互作用的要点。多巴胺的作用，以及 多巴胺和谷氨酸受体对细胞凋亡数量的影响 和/或坏死性细胞死亡也将由TUNEL技术确定。

DESCRIPTION: (Adapted from the Investigator's Abstract): Experimental models will induce carefully controlled levels of brain hypoxia, quantitated by direct measurement of oxygen levels in the tissue microcirculation, followed by periods of reoxygenation. These models will include brain hypoxia induced by arterial hypoxia (decreased FiO2; a high flow hypoxia) and hemorrhagic hypotension with bilateral carotid occlusion (a low flow hypoxia). The mechanism(s) of cellular dysfunction and/or injury will be examined at both the cellular and molecular levels. Changes in extracellular levels of glutamate and dopamine will be continuously monitored by in vivo microdialysis. The brain tissue will be screened for multiple genes that may be altered in expression level as the results of the hypoxic/ischemic episodes and reoxygenation. This will be performed using the amplified antisense RNA (aRNA) technique, and genes selected for analysis will correspond to proteins that are implicated as key points in dopamine-glutamate interactions. The role of dopamine, and of dopamine and glutamate receptors on the number of cells undergoing apoptosis and/or necrotic cell death will also be determined by the TUNEL technique.