Role and Regulation of Protein Phosphatase 5 in Brain

描述(由申请人提供)： 可逆的磷酸化是一种重要和常见的调节机制 脑的多种功能，包括电兴奋和巩固 对记忆的记忆。关于蛋白质的识别和调节，我们知道得很多。 参与这些过程的蛋白水解酶，但对 蛋白磷酸酶是信号中的蛋白激酶的专有伙伴 转导。我们的长期目标是了解它的功能和调节 Ser/Thr蛋白磷酸酶5在中枢神经系统中的表达。我们最近 从脑中提纯了这种酶，作为一种脂质激活的酶。尽管很少有 已知蛋白磷酸酶5的生物学功能，研究 非神经细胞已经将这种酶与激素信号通路联系在一起。 控制基因表达，离子通道调节和其他过程 是神经元功能的中心。为了理解这一角色 大脑中的酶，我们计划在神经元和 内分泌细胞。此外，我们将定义在其中的信号通路 蛋白磷酸酶5参与大脑，以进一步了解如何 荷尔蒙和神经递质控制神经功能。

DESCRIPTION (provided by applicant): Reversible phosphorylation is an important and common mechanism for regulating many functions in brain including electrical excitation and the consolidation of memory. Much is known concerning the identity and regulation of protein kinases that participate in these processes, but far less is known about the protein phosphatases that are the obligate partners of kinases in signal transduction. Our long-term goal is to understand the function and regulation of Ser/Thr protein phosphatase 5 in the central nervous system. We recently purified this enzyme as a lipid-activated enzyme from brain. Although little is known about the biological function of protein phosphatase 5, studies in non-neuronal cells have implicated this enzyme in hormone signaling pathways controlling gene expression, ion channel regulation and other processes that are central to the function of neurons. In order to understand the role of this enzyme in brain, we plan to identify its cellular targets in neurons and endocrine cells. In addition, we will define signaling pathways in which protein phosphatase 5 participates in brain to further our understanding of how hormones and neurotransmitters control neuronal function.