MOLECULAR BASIS FOR VERTEBRATE CELL MOTILITY

描述(申请者在具体目标中提供)：我们的长期目标是 了解非肌肉细胞是如何运动和分裂的。肌动蛋白和肌动蛋白发动机 为非肌肉细胞的运动和胞质分裂提供动力，以及一种 多种肌动蛋白结合蛋白调节这一过程。然而，这种方式 肌动蛋白结合蛋白是如何做到这一点的，目前还知之甚少。我的 主要的兴趣是原肌球蛋白亚型的作用和 与原肌球蛋白相互作用的蛋白质，包括钙调蛋白， 原调节蛋白、内皮抑素和肌球蛋白Va。在下一个项目期间，我将 继续探索这些分子如何惰性地影响细胞的运动和 细胞分裂素。 本项目旨在研究原肌球蛋白亚型及其亚型的作用。 调控非肌肉运动和胞质分裂的相互作用蛋白 细胞。这些拟议的研究将结合分子、生化和细胞 生物学方法与计算机辅助重建和 运动分析研究钙调蛋白的磷酸化功能 原肌球蛋白亚型、原肌球蛋白与原肌球蛋白的相互作用和 与内皮抑素和肌球蛋白Va的功能。

Description (provided in specific aims by applicant): Our long term goal is to understand how nonmuscle cells move and divide. Actin and actin motors provide the driving force for nonmuscle cell motility and cytokinesis, and a variety of actin-binding proteins regulate this process. However, the manner in which actin-binding proteins accomplish this is poorly understood. My major interest has been in the role of the tropomyosin isoforms and the proteins that interact with the tropomyosins, including caldesmon, tropomodulin, Endostatin and myosin Va. In the next Program period, I will continue to explore how these molecules ineract to effect cell motility and cytokineses. This project aims to study the roles of tropomyosin isoforms and their interacting proteins in regulating the motility and cytokinesis of nonmuscle cells. These proposed studies will combine molecular, biochemical and cell biological approaches together with computer-assisted reconstruction and motion analysis to investigate the functions of caldesmon phosphorylation sites, tropomyosin isoforms, tropomyosin interactions with tropomodulin and with endostatin, and functions of myosin Va.