CORE--TISSUE CULTURE/TISSUE RECONSTITUTION

描述：1.为研究人员提供细胞培养专业知识 计划项目。2.保持正常的成纤维细胞供应和 来自多种来源的角质形成细胞，冷冻保存在液氮中，用于 由核心B和其他项目使用。3.对皮肤细胞进行基因改造 使用Core C.提供的逆转录病毒载体。4.克隆和/或扩展 用于项目I、III、IV和项目实验的转基因细胞 五.维持转基因细胞的供应(正常和 不朽)，储存在液氮中，以供恢复和膨胀时使用 需要的。6.为项目4.7培养毛乳头细胞。7.当体积 项目IV中使用的质粒DNA的需求超过了核心C的能力 为了提供同样的物质，核心B将制备和纯化所需数量的质粒 DNA8.测试转基因细胞的转基因表达(蛋白质 产品和信息)，用于项目I、IV和V。9.基因嫁接 将适当结构中的皮肤细胞修饰到无菌小鼠身上 项目三.10.准备和维护去表皮化真皮(又称 脱细胞)用于项目I和V。11.移植四环素有反应 将Project V的永生化转基因角质形成细胞移植到裸鼠身上。 12.维护存储单元格的目录。

Description: 1. Provide cell culture expertise to investigators with the Program Project. 2. Maintain a supply of normal fibroblasts and keratinocytes from multiple sources, stored frozen in liquid nitrogen, for used by Core B and the other projects. 3. Genetically modify skin cells using retroviral vectors provided by Core C. 4. Clone and/or expand the genetically modified cells for used in experiments in Projects I, III, IV and V. 5. Maintain a supply of genetically modified cells (normal and immortalized), stored in liquid nitrogen for recovery and expansion when needed. 6. Culture dermal papillae cells for Project 4. 7. When volume needs for plasmid DNA for use in Project IV exceeds the capability of Core C to supply same, Core B will prepare and purify the required amounts of plasmid DNA. 8. Test genetically modified cells for transgene expression (protein product and message) in vitro for Projects I, IV and V. 9. Graft genetically modified cells of the skin in the appropriate constructs onto athymic mice for Project III. 10. Prepare and maintain de-epidermized dermic (also called acellular) for Projects I and V. 11. Transplant the tetracycline responsive immortalized genetically modified keratinocytes of Project V to nude mice. 12. Maintain a catalog of the stored cells.