HUMAN T CELLS ANTIGENS OF MYCOBACTERIUM TUBERCULOSIS

描述（改编自申请人的摘要）：本提案旨在 鉴定和表征M的T细胞抗原。结核病可能是 重要的候选疫苗。为了鉴定新的Mtb抗原， 与结核病的保护性免疫相关，我们使用了各种 抗原发现方法强调用抗M. 结核病抗血清和人T细胞。8种候选疫苗（Mtb 8.4，Mtb Mtb 9.8、Mtb 9.9、Mtb 11、Mtb 19、Mtb 32、Mtb 39、Mtb 40） 基于它们刺激来自感染M. 结核这8个抗原基因将作为重组基因进行评价。 抗原或作为裸DNA，单独或组合，用于免疫原性， 在包括食蟹猴在内的三种动物模型中对结核病的保护 猴子、老鼠和豚鼠。这些模型将用于评估T细胞 回应和保护。此外，我们将强调使用 人T细胞表达克隆以鉴定其他疫苗候选抗原。 这些将在上述体外和动物模型中表征。

DESCRIPTION (Adapted from the Applicant's Abstract): This proposal is aimed at identifying and characterizing T cell antigens of M. tuberculosis which may be important vaccine candidates. To identify novel Mtb antigens which may be relevant in protective immunity against tuberculosis we have used a variety of antigen discovery methods which emphasize expression cloning with anti-M. tuberculosis antisera and human T cells. Eight vaccine candidates (Mtb 8.4, Mtb 9.8, Mtb 9.9, Mtb 11, Mtb 19, Mtb 32, Mtb 39, Mtb 40) have so far been selected based on their ability to stimulate PBMC from donors infected with M. tuberculosis. These eight antigen genes will be evaluated as recombinant antigens or as naked DNA, alone or in combination, for immunogenicity and protection against tuberculosis in three animal models including cynomolgus monkeys, mice, and guinea pigs. These models will be used to assess T cell responses as well as protection. In addition, we will emphasize the use of human T cell expression cloning to identify other vaccine candidate antigens. These will be characterized in the in vitro and animal models as above.