HISTONE LIKE PROTEINS ASSOCIATED WITH KINETOPLAST DNA

描述(改编自申请者摘要)：原生动物为 内脏和皮肤利什曼病、昏睡病的病原体 以及热带和亚热带世界大部分地区的恰加斯病。这些 寄生生物以一种独特的线粒体DNA为特征 被称为动原体DNA或kDNA。这个DNA由几千个 小圆和大约25个最大圆，所有这些都交织成一个 凝聚成高度有序的盘状DNA网络的单DNA网络 鞭毛底部的结构。释放环状DNA分子 从网络单独复制，然后重新加入网络 最终会随着所有环状DNA分子成为 复制的。然后将双倍大小的网络分为两个和一个统一大小 网络在完成之前被划分为两个子小区中的每一个 细胞分裂。这项研究将调查以下群体的作用 组蛋白样动泡体蛋白在组织和分离中的作用 动质体中的kDNA。

DESCRIPTION (Adapted from the Applicant's Abstract): Kinetoplastid protozoa are the causative agents of visceral and cutaneous leishmaniasis, sleeping sickness and Chagas' disease in much of the tropical and subtropical world. These parasitic organisms are characterized by a unique form of mitochondrial DNA called a kineplast DNA or kDNA. This DNA consists of several thousand minicircles and about 25 maxicircles, all of which are interlocked into a single DNA network which is condensed into a highly organized disk-shaped structure at the base of the flagellum. Circular DNA molecules are released individually from the network, replicated and then rejoined to the network which eventually doubles in size as all circular DNA molecules become duplicated. The double sized network is then divided into two and a united size network is partitioned to each of two daughter cells prior to completion of cell division. This research will investigate the role of the group of histone-like kinetoplast proteins in the organization and segregation of the kDNA in the kinetoplastid Crithidia fasciculata.