ADVANCED MULTISPECTRAL IMAGING FOR MEDICAL DIAGNOSTICS

描述（申请人的摘要逐字）：该项目将开发一个 使用同步发光的新型多光谱成像（MSI）系统 (SL)概念，以快速检测体内癌症。该提案将解决 恶性肿瘤的实时体内识别和表征问题 以及上消化道的癌前组织。虽然存在 目前，Barrett粘膜的损伤很容易通过内窥镜检测到 通过广泛的活组织检查发现发育不良和早期癌症。典型协议 是在巴雷特粘膜上每隔2厘米做四个象限的活检虽然这 是标准技术，它只提供3- 5%的粘膜取样， 表面的发育不良和弥漫性癌可能会被发现。其余97-95 %的粘膜不取样。激光诱导荧光（LIF） 光谱学已经用于检测癌症和高度发育不良， 巴雷特食管。然而，该系统使用接触技术， 在每次测量时对1 mm区域的组织进行取样。虽然接触式LIF系统 比捏夹活检技术更好，需要一种新的系统来允许 检查粘膜的整个表面。为了满足这一重要需求， 在成像方面，将开发一种实时同步成像系统， 现有技术的声光可调谐滤波器技术耦合到 内窥镜将开发新的MSI成像技术， 恶性肿瘤与恶性肿瘤之间SL特性的微小差异的分辨率图像， 非恶性肿瘤。同步发光分析将大大简化 组织发出的荧光。这反过来又将提供一个更快的 并且无需活组织检查就能进行更准确的体内分析。独特的成像方面， 该MSI系统将提供实时空间信息， 对感兴趣的大区域进行全面诊断。随着 这项技术，初步研究将在两个模型系统上进行， 在橡树岭国家实验室， （ORNL）和田纳西大学。一旦系统被优化， 临床体内研究将在Thompson的人类受试者中进行。 田纳西州诺克斯维尔的癌症生存中心。一个跨学科 方法将用于执行拟议的研究，以提供结果， 高效和成本效益的方式。项目人员包括 跨学科研究人员小组，在ORNL独一无二， 在广泛的技术领域拥有丰富的经验：分子光谱学和 先进的仪器，分子生物学，细胞生物学。 微电子学、分子遗传学和小鼠研究。此外，本发明还提供了一种方法， 项目人员包括田纳西大学的兽医， 在TCSC具有临床研究专业知识的医学研究人员。

DESCRIPTION (Verbatim from Applicant's Abstract): This project will develop a novel multi-spectral imaging (MSI) system using the synchronous luminescence (SL) concept to rapidly detect cancer in vivo. The proposal will address the problem of real-time in vivo identification and characterization of malignant and pre-malignant tissues in the upper gastrointestinal tract. While presence of Barrett's mucosa is simple to detect endoscopically, at the present time dysplasia and early cancer is found by extensive biopsies. The typical protocol is four quadrant biopsies at 2-cm intervals of the Barrett's mucosa. While this is the standard technique, it only provides 3-5 percent sampling of the mucosal surface where dysplasia and diffuse cancer may be found. The remaining 97-95 percent of the mucosa is not sampled. Laser-induced fluorescence (LIF) spectroscopy has already been used to detect cancer and high grade dysplasia in Barrett's esophagus. However, that system uses a contact technique which samples a 1 mm area of tissue at each measurement. While the contact LIF system is better than the pinch biopsy technique, a new system is needed to allow examination of the entire surface of the mucosa. To address this important need in imaging, a real-time synchronous imaging system will be developed, based on state-of-the-art acousto-optic tunable filter technology coupled to an endoscope. Novel MSI imaging technology will be developed to obtain spatially resolved images of the slight differences in SL properties of malignant versus non-malignant tumors. Synchronous luminescence analysis will greatly simplify the resulting fluorescence from the tissue. This in turn will provide a faster and more accurate in vivo analysis without biopsy. The unique imaging aspect of this MSI system will provide real-time spatial information, allowing for comprehensive diagnosis of large areas of interest. Following development of this technology, initial studies will be performed on two model systems, biopsied tissues as well as laboratory animals at Oak Ridge National Laboratory (ORNL) and the University of Tennessee. Once the system has been optimized, clinical in vivo studies will be performed on human subjects at the Thompson Cancer Survival Center (TCSC) in Knoxville, Tennessee. An interdisciplinary approach will be used to perform the proposed research to provide results in an efficient and cost effective manner. Project personnel include an interdisciplinary group of researchers, uniquely available at ORNL, with experience in a wide range of technical areas: molecular spectroscopy and advanced instrumentation, molecular biology, cellular biology. microelectroptics and molecular genetics and mouse studies. In addition, project personnel include a veterinarian at the University of Tennessee, and medical researchers with expertise in clinical studies at the TCSC.