CORE--ENDOCRINOLOGY

在这个项目中，压力对伤口愈合的影响将在几个人类和动物模型中进行研究。将检查各种因变量，包括衰老、免疫、心理和社会心理变量以及脊髓损伤。压力会影响这些实体，而压力和这些变量之间最有可能的中介是神经内分泌系统。在每个项目中，下丘脑-垂体-肾上腺（HPA）轴将被检查以确定其在应激相关的伤口愈合抑制中的作用。血浆皮质醇和/或唾液皮质醇中发现的生物活性游离类固醇，以及ACTH和脱氢表雄酮将代表HPA系统的激素。此外，我们计划检查垂体生长激素和IGF-1，因为它们具有充分证明的免疫增强特性（Berczi, 1994）。Malarkey博士的内分泌实验室拥有运行本提案中概述的所有检测的专业知识。我们认为，不必在五个不同的实验室培训多名技术人员，一名全职技术人员可以提高这些检测的效率和准确性。因此，我们相信内分泌核心将产生成本节约，以及提高准确性的分析。内分泌核心将让内分泌学家马拉基博士密切参与这四个项目的内分泌影响，每个项目都有一个内分泌成分。

In this program project grant the influence of stress on wound healing will be examined in several human and animal models. Various dependent variables will be examined including aging, immunity, psychological and psychosocial variables, as well as spinal cord injury. Stress influences each of these entities and the most probably mediator between stress and these variables is the neuroendocrine system. In each project, the hypothalamic-pituitary-adrenal (HPA) axis will be examined to determine its role in the stress-related inhibition of wound healing. Plasma cortisol and/or the biologically-active free steroid found in salivary cortisol, as well as ACTH and DHEA will represent hormones of the HPA system. In addition we plan to examine pituitary growth hormone and IGF-1 because of their well-documented immune enhancing properties (Berczi, 1994). Dr Malarkey's endocrine lab has expertise in running all of the assays outlined in this proposal. In stead of having to train multiple technicians in five different laboratories, it is our opinion that one full-time technician can produce increased efficiency as well as accuracy in performance of these assays. Hence, we believe the endocrine core would produce cost savings as well as enhance accuracy of the assays. The endocrine core will keep Dr. Malarkey, the endocrinologist closely involved with the endocrine affects of the four projects, each of which has an endocrine component.