CORE--ANIMAL/MORPHOLOGY FACILITY

动物/形态核心B将为每个单独的项目提供SAMP1/Yit小鼠。在统一的环境条件下，将SAMP1/YIT繁殖群体和实验动物保持在共同的集中设施中对该计划的成功至关重要，因为有证据表明，环境辅助因素，如肠道细菌菌群的定植，会影响疾病的存在或病程。动物生产的集中化还将通过消除多余的繁殖群体来降低成本，并最终通过确保每个项目的有效利用来减少所需的动物数量。第二个目标是为每个项目提供集中的组织学服务和免疫组织化学、数字荧光和明场显微镜方面的援助。动物/形态学核心的第三个目标是向项目内的研究人员提供炎症和其他形态参数的集中组织病理学分析。这一模式中用于分析回肠炎组织病理学特征的中央核心组成部分将为解释四个项目中每一个项目产生的数据提供一个共同框架。这一部分的优势在于有两名经验丰富的胃肠道病理学家，Moskaluk博士和Nast博士，对每个项目进行组织病理学评分，并在该模型中就疾病的病理特征向项目提供持续的咨询服务。第四个目标是提供一种资源，为小鼠提供关于诱导突变的资源，在适当的背景下诱导突变可以在3到4代内完成。科恩博士将担任核心主任，莫斯卡卢克博士将担任联席主任。该计划的PI由核心主任、联席主任和科米内利博士组成的委员会将处理常规质量控制和优先事项问题。监督委员会将每年审查核心的运作情况。

The Animal/Morphology Core B will provide SAMP1/Yit mice for use in each of the individual projects. Maintaining the SAMP1/Yit breeding colony and experimental animals in a common centralized facility under uniform environmental conditions is crucial to the success of the program because of evidence that environmental cofactors such as colonization of the gut with bacteria flora can effect the presence or course of disease. Centralization of animal production will also reduce costs by eliminating redundant breeding colonies, and ultimately reduce the numbers of animals required by ensuring efficient utilization by each of the projects. A second aim is to provide centralized histological services and assistance with immunohistochemistry, digital fluorescence and brightfield microscopy for each of the projects. The third aim of the Animal /Morphology core is to provide centralized histopathological analyses of inflammation and other morphological parameters to investigators within the program. A centralized core component for analysis of the histopathological features of ileitis in this model will provide a common framework for interpretation of data generated by each of the four projects. A strength of this component is the availability of two experienced GI pathologists, Dr. Moskaluk and Dr. Nast, to perform histopathological scoring of samples to each of the projects and to provide ongoing consultative service to the projects concerning the pathological features of disease in this model. The fourth aim is to provide a resource for providing mice with induced mutations on induced mutations into an appropriate background can be accomplished in 3 to 4 generations. Dr. Cohn will direct the core and Dr. Moskaluk will serve as co-director. A committee consisting of the core director, the co-director, and Dr. Cominelli, the PI of the program will address routine quality control and prioritization issues. An oversight committee will review the operation of the core on yearly basis.